The Cover
Overexpressed human RAD50 induced endoreduplication in part of SaOS-2 cells. To visualize whole multinucleated giant cell, SaOS-2 cells transiently transfected with human RAD50 cDNA were analyzed by immunostaining and confocal laser scanning microscopy. For tubulin (red) staining, monoclonal mouse anti-&agr; tubulin antibody and Alexa Fluor 568-conjugated goat antimouse IgG antibody were used. The nucleus (green) was then counterstained with Sytox Green. It showed that overexpressed human RAD50 induced endoreduplication or nuclear fragmentation. For details, see the article by Shin et al. in this issue.