The Cover

Immunofluorescence of endogenous BAX and overproduced RERE protein in human neuroblastoma tumor cells (SK-N-AS). RERE is detected as a green fluorescent protein (GFP)-fusion protein (green, upper cell). BAX protein is immunochemically detected using commercial antibody B-9. The micrograph display highlights the cytoplasmic fraction of RERE which colocalizes with BAX in conspicuous aggregates, preferentially in proximity to the nucleus. Note also the nuclear fraction of RERE protein localized to promyelocytic leukemia Oncogenic Domains (PMLs). The colocalization of RERE and BAX is followed by dramatic morphologic changes, identical to apoptotic features. This effect is highly significant because GFP-RERE/RERE transfected cells display much higher percentages of apoptotic cells than GFP-only controls.