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Fig. 3. Quantification of increases in total and active K-ras p21 at postconfluent growth arrest. A, in quadruplicate samples, K-ras-GTP was compared at 50% versus 2 days postconfluent cultures (100%+). Average scan values were 2.1 ± 0.6 versus 429 ± 51, an average fold increase of 206.5. B, Erk1/2 protein levels were determined in the starting lysates used for A, above, to confirm equality. Average scan values for K-ras-GTP normalized to Erk1/2 protein were 1.1 ± 0.3 and 224 ± 26, an average fold increase of 203.6. C, for direct comparison of these samples, the following amounts of starting-lysate protein were loaded as indicated on the gels: 1 mg for K-ras-GTP, 50%; 500 µg for K-ras-GTP, 100%+; 40 µg for total K-ras p21, 50%; and 20 µg for total K-ras p21, 100%+. Average scan values were 4.2 ± 1.2, 411 ± 87, 573 ± 76, and 1171 ± 162. After correction for amounts of protein loaded, the relative amounts were 1:196:3411:13,940.





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HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation