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Fig. 4. The changes in expression of brachyury and Pax-6 in differentiating EC cells. Both A.1 and 1.3 EC cells were cultured for 48 h with and without 10-6 M RA and then lysed for RNA isolation. Equal amounts of total RNA were reverse transcribed, and the resulting cDNAs were PCR amplified using brachyury- and Pax-6-specific primer pairs. The resulting PCR products were separated on agarose gel and visualized by staining with ethidium bromide. Data are representative of at least three independent replicates.