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Fig. 3. Triple labeling of HeLa cells (AC) and human keratinocytes (DF) with antibody to topoisomerase II (A, D) antibody to topoisomerase IIß (B, E) and with the DNA stain DAPI (C, F). Among the interphase cells in both cultures, all nuclei are labeled with antibody to topoisomerase IIß. A subset of the interphase cells (open arrows) does not show labeling for topoisomerase II. Cells in M phase (closed arrows) show labeling of mitotic chromosomes with antibodies to topoisomerase II and topoisomerase IIß. Bar, 5 µm.