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Fig. 6. Expression of mRNA for endophilin-I and CtBP/BARS in Jurkat cells or in the sublines. Total RNA (1 µg) was obtained from Jurkat, Jurkat-ws, or Jurkat-hp cells, reversed transcribed, and the resulting cDNA subjected to PCR. Endophilin-I and CtBP/BARS fragments of 716 bp or 239 bp, respectively, were amplified and separated in agarose gels, as indicated. The same protocol was used to amplify a ß-actin fragment from the same samples, used as a loading control in the bottom panel. The experiment shown is representative of five different experiments.