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Fig. 1. Surface expression of Fas/CD95, CD3, and CD25 in parental Jurkat cells, and in the hyperproliferative sublines Jurkat-hp and Jurkat-ws. Jurkat (top panels), Jurkat-hp (middle panels), or Jurkat-ws cells (bottom panels) were stained at 4°C with FITC-labeled anti-Fas mAb SM1/23, anti-CD3 mAb OKT3, or anti-CD25 mAb 3G10, and the surface expression of the proteins analyzed by flow cytometry (black histograms). White histograms correspond to the staining of the same cells with an irrelevant mouse IgG coupled to the same fluorophore. Diagrams shown are representative of at least three different experiments performed for each experimental condition.