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Fig. 4. Effect of variable concentrations of FCS in the culture medium on cell numbers (A) and on Wt1 and ß-actin transcript levels (B). The Y-79 cells were suspended in 24-well plates at a density of 104 cells/ml and cultured for 3 days in RPMI 1640 containing the indicated concentration of FCS. Wt1 and ß-actin mRNA levels were measured by a semiquantitative RT-PCR. Note that Wt1 transcripts, unlike the cell proliferation rates reflected by the cell counts, did not change in proportion to the FCS concentration in the tissue culture medium. Bars, SD.