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Fig. 3. A, Western blot analysis of CDC34 and ß-actin expression in FACS-sorted normal resting T and B lymphocytes. B, Western blot analysis of CDC34, cyclin D3, and p27KIP1 expression in normal resting PBMCs stimulated with PHA. T0, cells at experiment initiation; C, control; S, suspension cells in the PHA-treated cells; A, adherent cells in the PHA-treated cells. The membrane was first probed with {alpha}-CDC34 antibodies and then reprobed with {alpha}-cyclin D3 antibodies and {alpha}-p27 antibodies without stripping. Therefore, the upper band in the middle panel represents residual staining for CDC34 protein.





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HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation