Fig. 3. A, Western blot analysis of CDC34 and ß-actin expression in FACS-sorted normal resting T and B lymphocytes. B, Western blot analysis of CDC34, cyclin D3, and p27KIP1 expression in normal resting PBMCs stimulated with PHA. T0, cells at experiment initiation; C, control; S, suspension cells in the PHA-treated cells; A, adherent cells in the PHA-treated cells. The membrane was first probed with -CDC34 antibodies and then reprobed with -cyclin D3 antibodies and -p27 antibodies without stripping. Therefore, the upper band in the middle panel represents residual staining for CDC34 protein.