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Fig. 2. PI3K signaling mediates specific expression of HIF-1{alpha} induced by insulin. PC-3 (A) and DU145 (B) cells were cultured without serum for 24 h. The PI3K-specific inhibitors LY294002 and wortmannin at the indicated concentrations were added 30 min before the addition of insulin. The cells were incubated in the presence (+) or absence (-) of insulin for 6 h. Nuclear extracts were prepared and used to analyze HIF-1{alpha} and HIF-1ß protein expression. The exposure times for immunoblots A and B were different, and the signals on these two blots are not comparable.





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HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation