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Fig. 1. Expression of HIF-1{alpha} is increased by the addition of insulin or with the mutation of PTEN in cancer cell lines. U2-OS, ACHN, DU145, AND PC-3 cells were cultured in serum-free MEM with Earle’s salts for 24 h, followed by incubation in the absence or presence of 200 nM insulin for 6 h. Nuclear extracts were prepared, and 10 µg of the nuclear extracts were used for immunoblot analysis and detected by antibodies against HIF-1{alpha} and HIF-1ß.





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HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation