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Fig. 2. The IFN-{gamma} pathway is not constitutively active in melanoma cell lines. Nuclear extracts were isolated from untreated melanoma cells and JKSF cells untreated or treated with 400 units/ml IFN-{gamma} for 2 h. Extracts were subjected to 10% SDS-PAGE. Immunoblotting was performed using an anti-IRF-1 antibody (Santa Cruz Biotechnology) and ECL. In vitro translated IRF-1 was used as a positive control for IRF-1 detection.





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HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation