CG&D
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation

Right arrow Help viewing high resolution images
Right arrow Return to article


Fig. 3. Stronger STAT activation in BW5147 cells overexpressing the IL-9R. BW5147 and BW-hIL9R cells were stimulated with murine IL-9 and hIL-9, respectively (500 units/ml), for the indicated periods of time. Alternatively, BW5147 cells were incubated with IFN-{gamma} (500 units/ml). Cells were lysed directly in sample buffer and loaded (105 cells/well) onto an 8% polyacrylamide gel. Phosphorylation of STAT1, STAT3, or STAT5 was visualized by Western blot with specific antibodies (New England Biolabs), according to the manufacturer’s instructions. As a control, blots were reprobed with an anti-actin antibody (data not shown). Similar results were obtained in three independent experiments.





Right arrow Return to article


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation