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Fig. 2. Effect of RhoGDI{alpha} on Rho family protein distribution in living cells. HeLa cells actively growing in medium containing 10% calf serum were transfected with plasmids containing GFP-tagged GTPase constructs in the absence or presence of a 3-fold excess of a RhoGDI{alpha} expression construct by LipofectAMINE PLUS reagent in serum-free medium, and 6 h posttransfection, cells were maintained in medium containing 10% serum. At 24 h posttransfection, cells were viewed with a Zeiss Axiophot fluorescence microscope, and digitized images were recorded.





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HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation