CG&D
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation

Right arrow Help viewing high resolution images
Right arrow Return to article


Fig. 6. Cytochrome c release is not the initiating event during IEC anoikis. IECs were processed at the indicated time points, and cytosolic and mitochondrial fractions were separated. A, IEC cytosol shows no cytochrome c (cyt. c) after isolation, and minimal amounts can be detected in the cytosol during the initial 30 min of anoikis (Western blot). However, cytochrome c is massively released after 60 min. Equal loading of cytosolic samples was demonstrated by probing the identical gel for actin. B, no detection of cytochrome c oxidase subunit IV demonstrated a lack of contamination by mitochondria in the cytosol by Western blot (c). Staining for cytochrome c oxidase subunit IV was positive in mitochondrial extracts (m), assuring us that purification of mitochondria was successful.





Right arrow Return to article


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation