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Fig. 2. After loss of cell anchorage, IECs rapidly undergo massive apoptosis. IECs were harvested and assessed for DNA content by PI staining. The cell aggregates remaining after a brief dispase treatment were identified and gated in the pulse width count. At the indicated times after loss of anchorage, PI staining of IECs was analyzed, showing a massive increase in the sub-G1 (M1) population of cells after 180 min undergoing anoikis, indicative of apoptosis.





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HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation