CG&D
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation

Right arrow Help viewing high resolution images
Right arrow Return to article


Fig. 1. Effect of PhIP on ß-casein promoter activity and gene expression in HC11 cells cultured with lactogenic hormones, DIP. Competent HC11-Lux cells were treated with PhIP in DM. A, concentration-dependent induction of luciferase activity in HC11-Lux cells treated with various concentrations of PhIP. B, induction of ß-casein promoter activity observed at various times after addition of 100 µM PhIP. In both A and B, the expression observed in the presence of PhIP in DM was compared with the expression observed in DM only. C, Northern blot analysis of ß-casein gene expression at various times after treatment with PhIP (100 µM) or in control (C) cells incubated in DM only. D, ethidium bromide-stained gel used for Northern blotting in panel C indicates equal loading of RNA in each lane. The ß-casein promoter activity was analyzed using the luciferase assay.





Right arrow Return to article


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation