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Table 2 Comparison of GnT-V mRNA expression in artificial and spontaneous hybrid cells of high and low metastatic potential as quantitated densitometry of gels

Values are expressed as ratios to 18S or 28S RNA. Results from two experiments are presented. Experiment 1 analyzed basal RNA levels with no MSH/IBMX treatments. Experiment 2 included MSH/IBMX treatment.

Cells Metastatic potentiala Ratio of relative band intensity

Experiment 1 (-MSH)

Experiment 2 (-MSH) (+MSH)

7.5 kb vs. 18S 3.5 kb vs. 18S 7.5 kb vs. 28S


Parental
    S91 0.14 0.78 1.08 0.07 0.16
    MØ n.a.b 2.12 2.53 n.d. n.d.
Hybrids
    95-H3 0 1.21 1.06 1.01 0.82
    95-H12 0.10 n.d. n.d. 0.91 1.39
    94-H4 0.43c 1.43 4.02 n.d. n.d.
    94-H48 0.49d 1.70 3.41 1.49 1.39
    95-H1 0.73d 3.62 1.87 1.43 1.82
    PADA 0.55d 3.89 3.33 5.29 2.84
    LM6e n.d. n.d. n.d. 1.64 2.42

a Metastatic potentials were determined previously (1) .

b n.a., not applicable; n.d., not done.

c P < 0.001.

d P < 0.0001 compared with parental Cloudman S91 melanoma cells.

e LM6, a clone from a spontaneous lung metastases, is highly motile in vitro (5) .





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