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Table 1 GnT-V activity in macrophage-fusion hybrid cells exhibiting high and low metastatic potential

Enzyme activity was measured using fluorescence-labeled sugar chain as substrate as described in "Materials and Methods." Values shown above are the average of four assays ± SD.

Cells Metastatic potentiala Enzyme activity (pmole/h/mg protein)

(no treatment) (+MSH/IBMX)

Parental cells
    S91 0.14 17.3 ± 2.4 21.5 ± 2.5
    MØ n.a.b 35.0 ± 5.2c n.d.
Hybrids
    95-H3 0 18.5 ± 1.9 18.6 ± 1.4
    95-H12 0.10 18.6 ± 1.5 21.2 ± 1.6
    94-H4 0.43c 28.5 ± 3.3c n.d.
    94-H48 0.49d 31.7 ± 1.8c 32.8 ± 1.4
    95-H1 0.73d 33.5 ± 4.1c 38.6 ± 3.1
    PADA 0.55d 41.1 ± 1.4c 41.0 ± 1.5
    LM6e n.d. 46.3 ± 2.8c 42.7 ± 2.4

a Metastatic potentials were determined previously (1) .

b n.a., not applicable; n.d., not done.

c P < 0.001.

d P < 0.0001 compared with parental Cloudman S91 melanoma cells.

e LM6, a clone from a spontaneous lung metastases, is highly motile in vitro (5) .





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