Fig. 2. Characterization of stably transfected SH-SY5Y cell clones.
A, Northern blot analysis of trkC expression in
SH-SY5Y/trkC cells. Fifteen µg of total RNA from
SH-SY5Y/wt, SH-SY5Y cells transfected with trkC (clones 3:1
and 3:2), with trkA (clone 6:2), or with empty
pMEXneo vector (clone 1:9) were transferred to the same filter
and probed for trkC and GAPDH expression, the
latter as an mRNA loading control. Bars at left,
approximate sizes of trkC transcripts (2.8 and 1.2 kb).
B, Northern blot analysis of the expression of the neuronal
marker genes, NPY (0.8 kb) and GAP-43 (1.4 and
1.6 kb) in differentiated SH-SY5Y/trkC (clone 3:1) and
SH-SY5Y/trkA cells. Cells were grown for 4 days in
serum-containing medium alone or in serum-containing medium
supplemented with 3 nM bFGF and 5 nM IGF-I or
with 16 nM TPA. Fifteen µg of total RNA of each sample
were electrophoretically separated and blotted onto the same filter.
GAPDH (1.5 kb) mRNA levels were used as a reference for the
amount of loaded RNA.