Fig. 6. Under reduced serum conditions, JunD levels increase in AKR-2B cells.
Subconfluent cultures of murine AKR-2B cells were incubated in growth
medium supplemented with 10% (Lanes 1, 3, 5, and
7) or 1% FBS (Lanes 2, 4, 6, and
8) for 1 day (Lanes 1 and
2), 2 days (Lanes 3 and
4), 3 days (Lanes 5 and
6), and 4 days (Lanes 7 and
8). Nuclear extracts were prepared, and extracts
containing equal amounts of proteins were analyzed by immunoblotting
using the anti-JunD antibody. The same membrane was subsequently probed
with antibodies to ß-actin (bottom panel) to control
loading of equal amounts of proteins in the lanes. An
arrow indicates the JunD protein band.