Fig. 4. IFN- induces increased p21 transcription in MCF-7 cells but not in
MDA-MB-231 cells. RNA from MCF-7 and MDA-MB-231 cells treated with and
without 10 ng/ml IFN- was collected, and p21 mRNA expression was
detected by RNase protection assay. RNA from A431 cells treated with
and without 30 ng/ml EGF was included as a positive control.
Hybridization to labeled probe representing 36B4 was used as a loading
control. p21 message was quantitated, and values for p21 divided by
36B4 are depicted as a bar graph. Data shown are representative of
three independent experiments.