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Fig. 2. CDK4 activity in vivo and in vitro. IP kinase assays were performed on whole-liver homogenates (A) and lysates of fetal hepatocytes cultured for the indicated times [B, E19 (filled bars) and E21 (unfilled bars)]. Activity was measured as incorporation of 32P from [{gamma}-32P]ATP into GST-pRb fusion protein. Representative autoradiograms are shown (lower panels) with corresponding densitometry (graphs). Error bars, SE. Results were confirmed in three additional experiments. C1, minus antibody control; C2, minus sample control; C3, minus substrate control.





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HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation