Fig. 1. Electroporation electrode and slide assembly. Cells are grown on glass
slides coated with conductive and transparent ITO within a "window"
cut into a Teflon frame as shown. The peptide solution is added to the
cells and introduced by an electrical pulse delivered through the
electrode set, which is placed directly on the frame. Two
configurations (A and B) were used.
A, partly conductive slide assembly, with electroporated
(a) and nonelectroporated (c) cells
growing on the same type of ITO-coated surface. b, area
where the conductive coating has been stripped, exposing the
nonconductive glass underneath. Cells growing in areas b
and c are not electroporated. B, fully
conductive slide assembly with a window of 32 x 10 mm (reprinted
from Ref. 23