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Table 1 Effect of PD098059 on cell proliferation in the presence of anti-IGF-IR antibody or isotype-matched control antibodya

No. of cells (x 104/cm2)

MCF-7 T-47D

Control+ DMSO 8.5 ± 0.9 7.5 ± 0.8
Anti-IGF-IR+ DMSO 8.0 ± 0.1 6.7 ± 0.6
Control+ PD098059 3.7 ± 0.5 3.1 ± 0.1
Anti-IGF-IR+ PD098059 5.5 ± 0.4 4.5 ± 0.4

a MCF-7 and T-47D cells were plated in triplicate in 24-well plates at densities of 1 x 104 and 2.5 x 104 cells/cm2, respectively. Cells subsequently were incubated in complete growth medium supplemented with: (a) control IgG isotype-matched antibody and DMSO vehicle control; (b) anti-IGF-IR antibody (clone {alpha}IR-3) and DMSO; (c) control IgG isotype-matched antibody and PD098059; or (d) anti-IGF-IR antibody (clone {alpha}IR-3) and PD098059. After 4 days, triplicate wells were counted for each treatment. Mean and SD are shown. Data are representative of repeated experiments. When included, 25 µM PD098059 and 400 ng/ml of antibodies were used.





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HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation