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Table 1 TTF1 transcriptional activity requires PKA

CAT expression driven by several promoter constructs in the presence of absence of PKA specific inhibitor (PKI) or its single-point mutant (PKI mut). +TTF1 + C-PKA indicates transfections, where TTF1 and PKA catalytic subunit expression vectors have been included. Sp1-CAT is a pEMBL8 plasmid containing the SV40 minimal promoter fused to the SP1 binding site derived from the ferritin promoter.

DNA % CAT activity

PKI PKI mut.

RSV-CAT 15 ± 2.5 16 ± 2 17 ± 3
pTgTTF1-CAT 1.2 ± 0.9 1.5 ± 1.2 1.6 ± 1.1
+ TTF1 4.5 ± 1.2 2.2 ± 1.5 4.2 ± 1.8
+ TTF1+ C-PKA 8.5 ± 2.1 3 ± 1.5 6.5 ± 1.9
Sp1-CAT 8.1 ± 1.8 7 ± 2.2 6.9 ± 2.5





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HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation