Fig. 2. PKA induction of TTF1 transcriptional activity in PKA- and
PKA+ cells. Increasing amounts of TTF1 cDNA plasmid were
transiently cotransfected with TTF1-CAT reporter cDNA in the presence
() or absence () of 2.5 µg of the C-PKA expression
vector in A126 (A) or COS7 (B).
Inset in A, CAT activity as function of
C-PKA plasmid concentration in the presence of 0.2 µg of the TTF1
expression plasmid. The results represent means of four independent
experiments and are expressed as a percentage of CAT enzymatic
activity; bars, SD.