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Fig. 1. Nonneuronal cells, but not neurons, proliferate and incorporate BrdU in dissociated cultures. A, BrdU-negative neuron (red arrow) in a dissociated DRG culture incubated with BrdU for 48 h appears more protuberant by DIC microscopy than nonneuronal cells (blue arrows), some of which are BrdU positive (brown). B, a BrdU-negative neuron (red arrow) is stained intensely for GAP-43 in both soma and processes (blue). In the same field are shown two nonneuronal cells (blue arrows), one of which is BrdU positive (brown). Bar: A and B, 20 µm. C, low magnification of 24–36 h cultures stained for GAP-43 using the 91E12 antibody. The antibody strongly labeled round cells with long processes that have been counted as neurons in the remainder of these studies (red arrows). The heterogeneous neurons in intact ganglia and in dissociated DRGs range in size from 5 to 50 µm in diameter. Flat cells with a shorter or absent processes that stained less intensely for GAP-43 were scored as nonneuronal cells (black arrows). Bar, 50 µm. D, prior sciatic nerve lesion (Ax.) and 10% horse serum (Ser) stimulated nonneuronal cell proliferation but was unable to stimulate DNA synthesis in neurons. Laminin and NGF were also unable to trigger BrdU incorporation in neurons. The data are expressed as means ± SD from four separate animals. All neurons and 400 nonneuronal cells in a 2-cm2 area were scored.





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HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation