Fig. 5. Cell death is independent of cdc25C function. A, cycling
and nocodazole-treated U-20S cells were treated with phosphatase,
and cdc25C mobility was determined by Western blotting.
I, interphase cdc25C. M, mitotic cdc25C.
B, SAOS-2 and U-20S cells were synchronized in
G1, infected at 100 and 30 MOI of dnPLK or control virus,
treated with nocodazole, and then harvested 24 h later for Western
blotting with cdc25C antibody. C, HMECs harvested after
48 h. D, MCF-7 cells infected with 650 and 300 MOI
and harvested after 24 h. Open arrow, mitotic form
of cdc25C. Solid arrow, interphase form of cdc25C.