Fig. 4. DN-PLK1 induces abnormal mitoses in SAOS-2 and U-20S but not in HMECs.
Cells were infected at 200 MOI of dnPLK1 and processed for
immunohistochemistry at different time points. SAOS-2 cells
immunostained 24 h postinfection for -tubulin
(A), -tubulin and DAPI (B), or
gamma-tubulin (C). Arrows, abnormal
prophase mitoses with disrupted mitotic spindles and poor centrosomal
-tubulin staining. U-2OS cells immunostained 48 h postinfection
for -tubulin (D). Arrows, abnormal
prophase and metaphase cells. HMECs were immunostained 48 h
postinfection with -tubulin and DAPI (E) or
-tubulin (F). Arrow, prophase cell with normal
monoastral spindle. Metaphase cell is just below.