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Table 2 Cell cycle distribution of ptsp53-expressing and mock-transfected U-937 cells

Cells were seeded in culture medium at 200,000 cells/ml and incubated at the indicated temperatures. On days 1, 2, and 3, cells were explored for cell cycle distribution by a FACS analysis. Values show percentage of viable cells (i.e., cells with sub-G1 DNA content are excluded). One representative experiment is shown.

Temperature Day 1

Day 2

Day 3

G0–G1 S-phase G2-M G0–G1 S-phase G2-M G0–G1 S-phase G2-M

U-937 M1 37°C 44.2 45.8 9.93 46.8 45.4 7.8 62.2 34.2 3.5
U-937/ptsp53/A2 55.2 31.6 13.2 53.2 41.3 5.5 55.5 39.8 4.7
U-937 M1 32.5°C 48.4 34.8 16.8 41.5 46.6 11.9 27.8 72.1 0.2
U-937/ptsp53/A2 76.6 16.2 7.2 87.2 5.1 7.7 96 4 0
U-937 M1 31.5°C 46.7 31.6 21.7 36.8 52.7 10.5 36.1 55.7 8.3
U-937/ptsp53/A2 76.1 6.55 19.4 85.4 6.5 8.1 86.5 11.7 1.8





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HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation