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Fig. 2. Temperature dependence of the levels of ptsp53-induced p21. The U-937/ptsp53 clone A2 and the mock-transfected U-937 clone M2 were incubated at 31.5°C, 32.5°C (i.e., temperatures permissive for wild-type p53 activity), and 37°C (i.e., the nonpermissive temperature for wild-type p53 activity). After 24 h, cells were subjected to Western blot using the mouse moAb anti-p21 WAF-1 Ab-1 and an actin antibody, serving as a control for equal loading (described in "Materials and Methods"). Arrows on the right, positions of the p21 and actin proteins. Left, positions of molecular weight standards (in thousands). *, the relative amount of p21 protein was estimated by densitometry as described in "Materials and Methods" and normalized to the amount of actin in the corresponding lane.





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HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation