Fig. 3. Expression of PPAR-1 and PPAR-2 isoforms in human prostate cancer
cell lines showing the effect of 15d-PGJ2 treatment.
a, cells were treated with 2.5 and 10 µM
15d-PGJ2 and harvested at 3-, 6-, and 24-h intervals.
Western blots were prepared and probed with anti-PPAR- antibody
(1:2000; BioMol), which detects both isoforms of PPAR-.
b, Western blots of benign (B) and
cancerous (T) prostate tissue, DU145 cells treated with
10 µM 15d-PGJ2 for 6 h, and
differentiated 3T3-L1 cells. The same antibody shown in
a was used in b.