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Fig. 2. Association between Mdm2 mutants and p53. Each of the indicated Mdm2 mutants (10 µg) was cotransfected into U2OS cells with wild-type p53 (10 µg). A, Western blot analysis of total p53 protein in lysates after cotransfection with Mdm2 and mutants. B, Western blot analysis of p53 protein coimmunoprecipitated from the same lysates as in A. Mdm2 proteins were immunoprecipitated using antibody SMP-14, and p53 was detected using rabbit serum CM-1. C, Western blot analysis of total Mdm2 protein in lysates after cotransfection with Mdm2 and mutants.





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HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation