Cell Growth & Differentiation, Vol 9, Issue 9 723-730, Copyright © 1998 by American Association of Cancer Research

ARTICLES

Basic fibroblast growth factor-induced decrease in type I collagen gene transcription is mediated by B-myb

KE Kypreos, MA Nugent and GE Sonenshein
Department of Biochemistry, Boston University School of Medicine, Massachusetts 02118, USA.

Basic fibroblast growth factor (bFGF), a member of the fibroblast growth factor family, potently induces increased vascular smooth muscle cell (SMC) proliferation and decreased expression of type I collagen. Recently, our laboratory demonstrated that, in bovine vascular SMCs, expression of B-myb, a member of the myb gene family, is dependent upon cellular growth state and that B-myb decreases alpha1(I) collagen promoter activity in transient transfection assays. Nuclear run-off analysis indicated that the decrease in alpha1(I) collagen mRNA level seen upon bFGF treatment was due to a decline in the rate of alpha1(I) procollagen gene transcription. Thus, we investigated the potential role of B-Myb in the down-regulation of type I collagen gene expression by bFGF. Using Northern blot analysis, we found that bFGF treatment of bovine aortic SMCs caused an increase in B-myb mRNA levels. Ectopic expression of B-myb decreased endogenous alpha1(I) collagen mRNA levels. Importantly, introduction of a B-myb antisense oligonucleotide prevented the drop in the alpha1(I) collagen mRNA levels seen upon treatment with bFGF. Together, these results indicate that B-myb mediates signals leading to the decreased rate of alpha1(I) collagen gene transcription caused by bFGF.

This article has been cited by other articles:

				C. S. Hofmann, X. Wang, C. P. Sullivan, P. Toselli, P. J. Stone, S. E. McLean, R. P. Mecham, B. M. Schreiber, and G. E. Sonenshein B-Myb Represses Elastin Gene Expression in Aortic Smooth Muscle Cells J. Biol. Chem., March 4, 2005; 280(9): 7694 - 7701. [Abstract] [Full Text] [PDF]

				C. S. Hofmann, C. P. Sullivan, H.-Y. Jiang, P. J. Stone, P. Toselli, E. D. Reis, I. Chereshnev, B. M. Schreiber, and G. E. Sonenshein B-Myb Represses Vascular Smooth Muscle Cell Collagen Gene Expression and Inhibits Neointima Formation After Arterial Injury Arterioscler. Thromb. Vasc. Biol., September 1, 2004; 24(9): 1608 - 1613. [Abstract] [Full Text] [PDF]

				C. B. Rich, I. Carreras, E. C. Lucey, J. A. Jaworski, J. A. Buczek-Thomas, M. A. Nugent, P. Stone, and J. A. Foster Transcriptional regulation of pulmonary elastin gene expression in elastase-induced injury Am J Physiol Lung Cell Mol Physiol, August 1, 2003; 285(2): L354 - L362. [Abstract] [Full Text] [PDF]

				M. M. Luchetti, P. Paroncini, P. Majlingova, J. Frampton, M. Mucenski, S. S. Baroni, P. Sambo, J. Golay, M. Introna, and A. Gabrielli Characterization of the c-Myb-responsive Region and Regulation of the Human Type I Collagen alpha 2 Chain Gene by c-Myb J. Biol. Chem., January 10, 2003; 278(3): 1533 - 1541. [Abstract] [Full Text] [PDF]

				A. K. Ghosh Factors Involved in the Regulation of Type I Collagen Gene Expression: Implication in Fibrosis Experimental Biology and Medicine, May 1, 2002; 227(5): 301 - 314. [Abstract] [Full Text] [PDF]