CG&D
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Itoh, H.
Right arrow Articles by Johnson, E. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Itoh, H.
Right arrow Articles by Johnson, E. M.

Cell Growth & Differentiation, Vol 9, Issue 8 651-665, Copyright © 1998 by American Association of Cancer Research


ARTICLES

Alterations in Pur(alpha) levels and intracellular localization in the CV-1 cell cycle

H Itoh, MJ Wortman, M Kanovsky, RR Uson, RE Gordon, N Alfano and EM Johnson
Department of Pathology, Mount Sinai School of Medicine, New York, New York 10029, USA.

Levels of the single-stranded DNA-binding protein Pur(alpha), previously implicated in control of both DNA replication and gene transcription, are altered during the CV-1 cell cycle. Just prior to the onset of S phase, Pur(alpha) levels drop precipitously, after which they recover nearly 8-fold throughout S and G2 to peak just after mitosis. As observed previously, Pur(alpha) binds the hypophosphorylated form of the retinoblastoma protein, Rb. Coimmunoprecipitation of Pur(alpha) and Rb reveals that the complex declines as cells enter S phase and does not reform as Pur(alpha) levels recover in S and G2. As Pur(alpha) levels recover, the protein is localized to nuclear foci containing newly replicated DNA, as determined by immunoelectron microscopy using different sized gold beads and antibodies against Pur(alpha) and bromodeoxyuridine-labeled DNA. These foci also contain cyclin A, and Pur(alpha) coimmunoprecipitates with cyclin A from extracts of cells in S and G2 phases. Pur(alpha) remains with these foci throughout G2, after the bulk of DNA synthesis has ceased. Changing levels of Pur(alpha) may affect Pur(alpha) functions at the onset of S phase and during progression to mitosis.


This article has been cited by other articles:


Home page
J Mol EndocrinolHome page
M. Shiota, A. Yokomizo, and S. Naito
Increased androgen receptor transcription: a cause of castration-resistant prostate cancer and a possible therapeutic target
J. Mol. Endocrinol., July 12, 2011; 47(1): R25 - R41.
[Abstract] [Full Text] [PDF]


Home page
Am. J. Physiol. Endocrinol. Metab.Home page
S. Zhao, R. J. Kelm Jr., and R. D. Fernald
Regulation of gonadotropin-releasing hormone-1 gene transcription by members of the purine-rich element-binding protein family
Am J Physiol Endocrinol Metab, March 1, 2010; 298(3): E524 - E533.
[Abstract] [Full Text] [PDF]


Home page
Cancer Res.Home page
L. G. Wang, E. M. Johnson, Y. Kinoshita, J. S. Babb, M. T. Buckley, L. F. Liebes, J. Melamed, X.-M. Liu, R. Kurek, L. Ossowski, et al.
Androgen Receptor Overexpression in Prostate Cancer Linked to Pur{alpha} Loss from a Novel Repressor Complex
Cancer Res., April 15, 2008; 68(8): 2678 - 2688.
[Abstract] [Full Text] [PDF]


Home page
J Biol ChemHome page
A. M. Knapp, J. E. Ramsey, S.-X. Wang, A. R. Strauch, and R. J. Kelm Jr.
Structure-Function Analysis of Mouse Pur II: CONFORMATION ALTERING MUTATIONS DISRUPT SINGLE-STRANDED DNA AND PROTEIN INTERACTIONS CRUCIAL TO SMOOTH MUSCLE {alpha}-ACTIN GENE REPRESSION
J. Biol. Chem., December 7, 2007; 282(49): 35899 - 35909.
[Abstract] [Full Text] [PDF]


Home page
J Biol ChemHome page
J. E. Ramsey, M. A. Daugherty, and R. J. Kelm Jr.
Hydrodynamic Studies on the Quaternary Structure of Recombinant Mouse Purbeta
J. Biol. Chem., January 19, 2007; 282(3): 1552 - 1560.
[Abstract] [Full Text] [PDF]


Home page
J Biol ChemHome page
A. M. Knapp, J. E. Ramsey, S.-X. Wang, K. E. Godburn, A. R. Strauch, and R. J. Kelm Jr.
Nucleoprotein Interactions Governing Cell Type-dependent Repression of the Mouse Smooth Muscle {alpha}-Actin Promoter by Single-stranded DNA-binding Proteins Pur{alpha} and Purbeta
J. Biol. Chem., March 24, 2006; 281(12): 7907 - 7918.
[Abstract] [Full Text] [PDF]


Home page
J Biol ChemHome page
S.-X. Wang, P. K. Elder, Y. Zheng, A. R. Strauch, and R. J. Kelm Jr.
Cell Cycle-mediated Regulation of Smooth Muscle {alpha}-Actin Gene Transcription in Fibroblasts and Vascular Smooth Muscle Cells Involves Multiple Adenovirus E1A-interacting Cofactors
J. Biol. Chem., February 18, 2005; 280(7): 6204 - 6214.
[Abstract] [Full Text] [PDF]


Home page
J. Neurosci.Home page
R. M. Miller, L. M. Callahan, C. Casaceli, L. Chen, G. L. Kiser, B. Chui, T. M. Kaysser-Kranich, T. J. Sendera, C. Palaniappan, and H. J. Federoff
Dysregulation of Gene Expression in the 1-Methyl-4-Phenyl-1,2,3,6-Tetrahydropyridine-Lesioned Mouse Substantia Nigra
J. Neurosci., August 25, 2004; 24(34): 7445 - 7454.
[Abstract] [Full Text] [PDF]


Home page
J Biol ChemHome page
M. Gupta, V. Sueblinvong, J. Raman, V. Jeevanandam, and M. P. Gupta
Single-stranded DNA-binding Proteins PUR{alpha} and PUR{beta} Bind to a Purine-rich Negative Regulatory Element of the {alpha}-Myosin Heavy Chain Gene and Control Transcriptional and Translational Regulation of the Gene Expression: IMPLICATIONS IN THE REPRESSION OF {alpha}-MYOSIN HEAVY CHAIN DURING HEART FAILURE
J. Biol. Chem., November 7, 2003; 278(45): 44935 - 44948.
[Abstract] [Full Text] [PDF]


Home page
J Biol ChemHome page
R. J. Kelm Jr., S.-X. Wang, J. A. Polikandriotis, and A. R. Strauch
Structure/Function Analysis of Mouse Pur{beta}, a Single-stranded DNA-binding Repressor of Vascular Smooth Muscle {alpha}-Actin Gene Transcription
J. Biol. Chem., October 3, 2003; 278(40): 38749 - 38757.
[Abstract] [Full Text] [PDF]


Home page
Mol. Cell. Biol.Home page
K. Khalili, L. Del Valle, V. Muralidharan, W. J. Gault, N. Darbinian, J. Otte, E. Meier, E. M. Johnson, D. C. Daniel, Y. Kinoshita, et al.
Pur{alpha} Is Essential for Postnatal Brain Development and Developmentally Coupled Cellular Proliferation As Revealed by Genetic Inactivation in the Mouse
Mol. Cell. Biol., October 1, 2003; 23(19): 6857 - 6875.
[Abstract] [Full Text] [PDF]


Home page
J Biol ChemHome page
L. E. Carlini, M. J. Getz, A. R. Strauch, and R. J. Kelm Jr.
Cryptic MCAT Enhancer Regulation in Fibroblasts and Smooth Muscle Cells: SUPPRESSION OF TEF-1 MEDIATED ACTIVATION BY THE SINGLE-STRANDED DNA-BINDING PROTEINS, Pur{alpha}, Pur{beta}, and MSY1
J. Biol. Chem., March 8, 2002; 277(10): 8682 - 8692.
[Abstract] [Full Text] [PDF]


Home page
Mol. Cell. Biol.Home page
D. M. Cimbora, D. Schubeler, A. Reik, J. Hamilton, C. Francastel, E. M. Epner, and M. Groudine
Long-Distance Control of Origin Choice and Replication Timing in the Human beta -Globin Locus Are Independent of the Locus Control Region
Mol. Cell. Biol., August 1, 2000; 20(15): 5581 - 5591.
[Abstract] [Full Text] [PDF]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation
Copyright © 1998 by the American Association of Cancer Research.