Cell Growth & Differentiation, Vol 9, Issue 8 611-617, Copyright © 1998 by American Association of Cancer Research

ARTICLES

Nitric oxide-induced apoptosis in human pancreatic carcinoma cell lines is associated with a G1-arrest and an increase of the cyclin-dependent kinase inhibitor p21WAF1/CIP1

S Gansauge, AK Nussler, HG Beger and F Gansauge
Department of General Surgery, University of Ulm, Germany.

Nitric oxide (NO) is a messenger molecule with various biological activities including DNA damage. In the present study, we examined the influence of endogenously produced NO on human pancreatic cell lines. In response to cytokine stimulation (tumor necrosis factor alpha, IFN-gamma, and interleukin 1beta), human pancreatic carcinoma cell lines expressed the inducible NO synthase that synthesizes NO, detectable as nitrate and nitrite in the culture supernatants. Endogenously produced NO induced apoptosis in all of the tested pancreatic carcinoma cell lines. In cell cycle analysis, endogenous production of NO revealed a G1-arrest in all of the tested cell lines. This G1-arrest was blockable by addition of NG-monomethyl-L-arginine. These data indicate that NO induces a G1-arrest followed by apoptosis in pancreatic carcinoma cell lines.

This article has been cited by other articles:

				S. Huguenin, F. Vacherot, L. Kheuang, J. Fleury-Feith, M.-C. Jaurand, M. Bolla, J.-P. Riffaud, and D. K. Chopin Antiproliferative effect of nitrosulindac (NCX 1102), a new nitric oxide-donating non-steroidal anti-inflammatory drug, on human bladder carcinoma cell lines Mol. Cancer Ther., March 1, 2004; 3(3): 291 - 298. [Abstract] [Full Text]

				P. Monti, B. E. Leone, F. Marchesi, G. Balzano, A. Zerbi, F. Scaltrini, C. Pasquali, G. Calori, F. Pessi, C. Sperti, et al. The CC Chemokine MCP-1/CCL2 in Pancreatic Cancer Progression: Regulation of Expression and Potential Mechanisms of Antimalignant Activity Cancer Res., November 1, 2003; 63(21): 7451 - 7461. [Abstract] [Full Text] [PDF]

				T.-A. Kim, H. K. Avraham, Y.-H. Koh, S. Jiang, I.-W. Park, and S. Avraham HIV-1 Tat-Mediated Apoptosis in Human Brain Microvascular Endothelial Cells J. Immunol., March 1, 2003; 170(5): 2629 - 2637. [Abstract] [Full Text] [PDF]

				S. Ekmekcioglu, J. A. Ellerhorst, J. B. Mumm, M. Zheng, L. Broemeling, V. G. Prieto, A. L. Stewart, A. M. Mhashilkar, S. Chada, and E. A. Grimm Negative Association of Melanoma Differentiation-associated Gene (mda-7) and Inducible Nitric Oxide Synthase (iNOS) in Human Melanoma: MDA-7 Regulates iNOS Expression in Melanoma Cells Mol. Cancer Ther., January 1, 2003; 2(1): 9 - 17. [Abstract] [Full Text] [PDF]

				K. Kashfi, Y. Ryann, L. L. Qiao, J. L. Williams, J. Chen, P. del Soldato, F. Traganos, and B. Rigas Nitric Oxide-Donating Nonsteroidal Anti-Inflammatory Drugs Inhibit the Growth of Various Cultured Human Cancer Cells: Evidence of a Tissue Type-Independent Effect J. Pharmacol. Exp. Ther., December 1, 2002; 303(3): 1273 - 1282. [Abstract] [Full Text] [PDF]

				K. M. Anderson and J. E. Harris Selected Features of Nonendocrine Pancreatic Cancer Experimental Biology and Medicine, June 1, 2001; 226(6): 521 - 537. [Abstract] [Full Text] [PDF]

				O. Salvucci, M. Carsana, I. Bersani, G. Tragni, and A. Anichini Antiapoptotic Role of Endogenous Nitric Oxide in Human Melanoma Cells Cancer Res., January 1, 2001; 61(1): 318 - 326. [Abstract] [Full Text]

				D. Champlin and J. Truman Ecdysteroid coordinates optic lobe neurogenesis via a nitric oxide signaling pathway Development, January 8, 2000; 127(16): 3543 - 3551. [Abstract] [PDF]

				H. Matsumoto, S. Hayashi, M. Hatashita, K. Ohnishi, T. Ohtsubo, R. Kitai, H. Shioura, T. Ohnishi, and E. Kano Nitric Oxide Is an Initiator of Intercellular Signal Transduction for Stress Response after Hyperthermia in Mutant p53 Cells of Human Glioblastoma Cancer Res., July 1, 1999; 59(13): 3239 - 3244. [Abstract] [Full Text] [PDF]