CG&D
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Masuda, M.
Right arrow Articles by Yoshikura, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Masuda, M.
Right arrow Articles by Yoshikura, H.

Cell Growth & Differentiation, Vol 9, Issue 5 381-391, Copyright © 1998 by American Association of Cancer Research


ARTICLES

Effects of procollagen C-proteinase enhancer protein on the growth of cultured rat fibroblasts revealed by an excisable retroviral vector

M Masuda, H Igarashi, M Kano and H Yoshikura
Department of Microbiology, Graduate School of Medicine, The University of Tokyo, Japan. mmasuda@m.u-tokyo.ac.jp

An excisable retroviral vector, TSN-lox, was developed by exploiting Cre-loxP homologous recombination. An integrated TSN-lox provirus could be excised, leaving a solo long terminal repeat at the integration site; inverse PCR, taking advantage of the solo long terminal repeat, was used to characterize cellular flanking sequences. A TSN-lox-transduced Rat2 cell clone, lox-7, was found to harbor the provirus in an intron of the procollagen C-proteinase enhancer protein (PCPE) gene, whose expression was lowered compared with that of the parental Rat2. When the vector provirus in lox-7 cells was excised, PCPE expression was elevated. The level of PCPE expression seemed to affect cell growth properties such as morphology, contact inhibition, and anchorage-independent growth. These results suggested that the excisable retroviral vector may be useful for studying the molecular basis for proviral insertion mutagenesis, and that PCPE may play a significant role in controlling cell growth and differentiation.


This article has been cited by other articles:


Home page
Biochem. J.Home page
R. Salza, F. Peysselon, E. Chautard, C. Faye, L. Moschcovich, T. Weiss, L. Perrin-Cocon, V. Lotteau, E. Kessler, and S. Ricard-Blum
Extended interaction network of procollagen C-proteinase enhancer-1 in the extracellular matrix
Biochem. J., January 1, 2014; 457(1): 137 - 149.
[Abstract] [Full Text] [PDF]


Home page
Mol. Cell. Biol.Home page
B. M. Steiglitz, J. M. Kreider, E. P. Frankenburg, W. N. Pappano, G. G. Hoffman, J. A. Meganck, X. Liang, M. Hook, D. E. Birk, S. A. Goldstein, et al.
Procollagen C Proteinase Enhancer 1 Genes Are Important Determinants of the Mechanical Properties and Geometry of Bone and the Ultrastructure of Connective Tissues
Mol. Cell. Biol., January 1, 2006; 26(1): 238 - 249.
[Abstract] [Full Text] [PDF]


Home page
J Biol ChemHome page
S. Bernocco, B. M. Steiglitz, D. I. Svergun, M. V. Petoukhov, F. Ruggiero, S. Ricard-Blum, C. Ebel, C. Geourjon, G. Deleage, B. Font, et al.
Low Resolution Structure Determination Shows Procollagen C-Proteinase Enhancer to be an Elongated Multidomain Glycoprotein
J. Biol. Chem., February 28, 2003; 278(9): 7199 - 7205.
[Abstract] [Full Text] [PDF]


Home page
J Biol ChemHome page
B. M. Steiglitz, D. R. Keene, and D. S. Greenspan
PCOLCE2 Encodes a Functional Procollagen C-Proteinase Enhancer (PCPE2) That Is a Collagen-binding Protein Differing in Distribution of Expression and Post-translational Modification from the Previously Described PCPE1
J. Biol. Chem., December 20, 2002; 277(51): 49820 - 49830.
[Abstract] [Full Text] [PDF]


Home page
J Biol ChemHome page
S. Ricard-Blum, S. Bernocco, B. Font, C. Moali, D. Eichenberger, J. Farjanel, E. R. Burchardt, M. van der Rest, E. Kessler, and D. J. S. Hulmes
Interaction Properties of the Procollagen C-proteinase Enhancer Protein Shed Light on the Mechanism of Stimulation of BMP-1
J. Biol. Chem., September 13, 2002; 277(37): 33864 - 33869.
[Abstract] [Full Text] [PDF]


Home page
J. Virol.Home page
Y. F. Jin, T. Ishibashi, A. Nomoto, and M. Masuda
Isolation and Analysis of Retroviral Integration Targets by Solo Long Terminal Repeat Inverse PCR
J. Virol., June 1, 2002; 76(11): 5540 - 5547.
[Abstract] [Full Text] [PDF]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation
Copyright © 1998 by the American Association of Cancer Research.