CG&D
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Cho, Y.
Right arrow Articles by Talmage, D. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cho, Y.
Right arrow Articles by Talmage, D. A.

Cell Growth & Differentiation, Vol 9, Issue 2 147-154, Copyright © 1998 by American Association of Cancer Research


ARTICLES

Distinct functions of protein kinase Calpha and protein kinase Cbeta during retinoic acid-induced differentiation of F9 cells

Y Cho, MG Klein and DA Talmage
Institute of Human Nutrition, Columbia University, New York, New York 10032, USA.

As F9 embryonal carcinoma cells differentiate into parietal endoderm-like cells, expression of conventional protein kinase C (PKC) changes. Undifferentiated stem cells express PKCbeta but not PKCalpha, whereas differentiated parietal endoderm cells express PKCalpha but not PKCbeta. To determine whether changes in PKCalpha and/or PKCbeta expression control retinoic acid (RA)- and dibutyryl cyclic AMP-induced F9 cell differentiation, we established cell lines stably expressing PKCalpha, PKCbeta, antisense PKCalpha, or antisense PKCbeta RNAs. Constitutive expression of PKCalpha or inhibition of PKCbeta expression in F9 stem cells enhanced RA induced differentiation, both by increasing total expression and accelerating RA-induced expression of laminins A, B1, B2, and type IV collagen. In addition, expressing PKCbeta in a parietal endoderm cell line caused these cells to retrodifferentiate into stem cells. Based on these results, we conclude that PKCbeta and PKCalpha are key targets for RA-regulated gene expression, that PKCalpha plays an important, active role in inducing and maintaining the parietal endoderm phenotype, and that PKCbeta activity is incompatible with maintaining the differentiated state of these cells.


This article has been cited by other articles:


Home page
J. Neurosci.Home page
Y. Chen, M. L. Hancock, L. W. Role, and D. A. Talmage
Intramembranous Valine Linked to Schizophrenia Is Required for Neuregulin 1 Regulation of the Morphological Development of Cortical Neurons
J. Neurosci., July 7, 2010; 30(27): 9199 - 9208.
[Abstract] [Full Text] [PDF]


Home page
J Biol ChemHome page
T. Yamasaki, A. Takahashi, J. Pan, N. Yamaguchi, and K. K. Yokoyama
Phosphorylation of Activation Transcription Factor-2 at Serine 121 by Protein Kinase C Controls c-Jun-mediated Activation of Transcription
J. Biol. Chem., March 27, 2009; 284(13): 8567 - 8581.
[Abstract] [Full Text] [PDF]


Home page
Eur J EndocrinolHome page
D. Pasquali, P. Chieffi, W. J Deery, G. Nicoletti, A. Bellastella, and A. A Sinisi
Differential effects of all-trans-retinoic acid (RA) on Erk1/2 phosphorylation and cAMP accumulation in normal and malignant human prostate epithelial cells: Erk1/2 inhibition restores RA-induced decrease of cell growth in malignant prostate cells
Eur. J. Endocrinol., April 1, 2005; 152(4): 663 - 669.
[Abstract] [Full Text] [PDF]


Home page
J. Pharmacol. Exp. Ther.Home page
A. Banan, L. J. Zhang, A. Farhadi, J. Z. Fields, M. Shaikh, C. B. Forsyth, S. Choudhary, and A. Keshavarzian
Critical Role of the Atypical {lambda} Isoform of Protein Kinase C (PKC-{lambda}) in Oxidant-Induced Disruption of the Microtubule Cytoskeleton and Barrier Function of Intestinal Epithelium
J. Pharmacol. Exp. Ther., February 1, 2005; 312(2): 458 - 471.
[Abstract] [Full Text] [PDF]


Home page
Am. J. Physiol. Cell Physiol.Home page
A. Banan, L.J. Zhang, M. Shaikh, J.Z. Fields, A. Farhadi, and A. Keshavarzian
{theta}-Isoform of PKC is required for alterations in cytoskeletal dynamics and barrier permeability in intestinal epithelium: a novel function for PKC-{theta}
Am J Physiol Cell Physiol, July 1, 2004; 287(1): C218 - C234.
[Abstract] [Full Text] [PDF]


Home page
Am. J. Physiol. Cell Physiol.Home page
A. Banan, L. J. Zhang, A. Farhadi, J. Z. Fields, M. Shaikh, and A. Keshavarzian
PKC-{beta}1 isoform activation is required for EGF-induced NF-{kappa}B inactivation and I{kappa}B{alpha} stabilization and protection of F-actin assembly and barrier function in enterocyte monolayers
Am J Physiol Cell Physiol, March 1, 2004; 286(3): C723 - C738.
[Abstract] [Full Text] [PDF]


Home page
J. Pharmacol. Exp. Ther.Home page
A. Banan, J. Z. Fields, L. J. Zhang, M. Shaikh, A. Farhadi, and A. Keshavarzian
{zeta} Isoform of Protein Kinase C Prevents Oxidant-Induced Nuclear Factor-{kappa}B Activation and I-{kappa}B{alpha} Degradation: A Fundamental Mechanism for Epidermal Growth Factor Protection of the Microtubule Cytoskeleton and Intestinal Barrier Integrity
J. Pharmacol. Exp. Ther., October 1, 2003; 307(1): 53 - 66.
[Abstract] [Full Text] [PDF]


Home page
J. Pharmacol. Exp. Ther.Home page
A. Banan, A. Farhadi, J. Z. Fields, L. J. Zhang, M. Shaikh, and A. Keshavarzian
The delta -Isoform of Protein Kinase C Causes Inducible Nitric-Oxide Synthase and Nitric Oxide Up-Regulation: Key Mechanism for Oxidant-Induced Carbonylation, Nitration, and Disassembly of the Microtubule Cytoskeleton and Hyperpermeability of Barrier of Intestinal Epithelia
J. Pharmacol. Exp. Ther., May 1, 2003; 305(2): 482 - 494.
[Abstract] [Full Text] [PDF]


Home page
J. Pharmacol. Exp. Ther.Home page
A. Banan, J. Z. Fields, A. Farhadi, D. A. Talmage, L. Zhang, and A. Keshavarzian
Activation of delta -Isoform of Protein Kinase C Is Required for Oxidant-Induced Disruption of Both the Microtubule Cytoskeleton and Permeability Barrier of Intestinal Epithelia
J. Pharmacol. Exp. Ther., October 1, 2002; 303(1): 17 - 28.
[Abstract] [Full Text] [PDF]


Home page
Am. J. Physiol. Gastrointest. Liver Physiol.Home page
A. Banan, L. Zhang, J. Z. Fields, A. Farhadi, D. A. Talmage, and A. Keshavarzian
PKC-zeta prevents oxidant-induced iNOS upregulation and protects the microtubules and gut barrier integrity
Am J Physiol Gastrointest Liver Physiol, October 1, 2002; 283(4): G909 - G922.
[Abstract] [Full Text] [PDF]


Home page
J. Pharmacol. Exp. Ther.Home page
A. Banan, J. Z. Fields, A. Farhadi, D. A. Talmage, L. Zhang, and A. Keshavarzian
The beta 1 Isoform of Protein Kinase C Mediates the Protective Effects of Epidermal Growth Factor on the Dynamic Assembly of F-Actin Cytoskeleton and Normalization of Calcium Homeostasis in Human Colonic Cells
J. Pharmacol. Exp. Ther., June 1, 2002; 301(3): 852 - 866.
[Abstract] [Full Text] [PDF]


Home page
Am. J. Physiol. Gastrointest. Liver Physiol.Home page
A. Banan, J. Z. Fields, D. A. Talmage, Y. Zhang, and A. Keshavarzian
PKC-{beta}1 mediates EGF protection of microtubules and barrier of intestinal monolayers against oxidants
Am J Physiol Gastrointest Liver Physiol, September 1, 2001; 281(3): G833 - G847.
[Abstract] [Full Text] [PDF]


Home page
Am. J. Physiol. Gastrointest. Liver Physiol.Home page
A. Banan, J. Z. Fields, D. A. Talmage, L. Zhang, and A. Keshavarzian
PKC-zeta is required in EGF protection of microtubules and intestinal barrier integrity against oxidant injury
Am J Physiol Gastrointest Liver Physiol, May 1, 2002; 282(5): G794 - G808.
[Abstract] [Full Text] [PDF]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation
Copyright © 1998 by the American Association of Cancer Research.