CG&D
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by deFazio, A.
Right arrow Articles by Sutherland, R. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by deFazio, A.
Right arrow Articles by Sutherland, R. L.

Cell Growth & Differentiation, Vol 8, Issue 8 903-911, Copyright © 1997 by American Association of Cancer Research


ARTICLES

Antisense estrogen receptor RNA expression increases epidermal growth factor receptor gene expression in breast cancer cells

A deFazio, YE Chiew, M McEvoy, CK Watts and RL Sutherland
Cancer Research Program, Garvan Institute of Medical Research, St. Vincent's Hospital, Sydney, New South Wales, Australia.

In human breast cancer, progression to a more malignant phenotype is often accompanied by decreased expression of estrogen receptor (ER) and increased expression of epidermal growth factor receptor (EGFR). Higher levels of this receptor tyrosine kinase are found in tumors lacking ER, and a quantitative, inverse relationship exists between the level of ER and EGFR mRNA in human breast cell lines. Antisense ER (ASER) RNA was used to evaluate the consequence of decreased ER expression in breast cancer cells, specifically to determine whether ER is involved in the regulation of EGFR gene expression. ER-positive MCF-7 human breast cancer cells were transfected with ASER, and clones constitutively expressing ASER RNA had decreased ER and up to a 3-fold increase in the expression of EGFR mRNA. To confirm that this observation was a direct consequence of ASER expression, a metal-inducible ASER expression construct was transfected into MCF-7 cells, and transfected clones were isolated and characterized. Northern analysis revealed an induction of ASER RNA within 1 h of the addition of zinc, which was followed by a 4-fold increase in EGFR mRNA levels, maximal at 6-12 h. The basal level of expression of the glucocorticoid receptor is also inversely related to that of ER among breast cancer cell lines, but neither constitutive nor inducible expression of ASER affected the expression of glucocorticoid receptor. These data support the hypothesis that the level of expression of ER specifically influences the expression of EGFR in human breast cancer cells and provides a potential link between loss of steroid sensitivity and the acquisition of autonomous growth.


This article has been cited by other articles:


Home page
Cancer Res.Home page
X. Qi, J. Tang, M. Loesch, N. Pohl, S. Alkan, and G. Chen
p38{gamma} Mitogen-Activated Protein Kinase Integrates Signaling Crosstalk between Ras and Estrogen Receptor to Increase Breast Cancer Invasion.
Cancer Res., August 1, 2006; 66(15): 7540 - 7547.
[Abstract] [Full Text] [PDF]


Home page
Endocr Relat CancerHome page
R I Nicholson, C Staka, F Boyns, I R Hutcheson, and J M W Gee
Growth factor-driven mechanisms associated with resistance to estrogen deprivation in breast cancer: new opportunities for therapy
Endocr. Relat. Cancer, December 1, 2004; 11(4): 623 - 641.
[Abstract] [Full Text] [PDF]


Home page
Clin. Cancer Res.Home page

Clin. Cancer Res., January 1, 2004; 10(1): 346s - 354s.



Home page
Cancer Res.Home page
D. K. Biswas, K. J. Martin, C. McAlister, A. P. Cruz, E. Graner, S.-c. Dai, and A. B. Pardee
Apoptosis Caused by Chemotherapeutic Inhibition of Nuclear Factor-{kappa}B Activation
Cancer Res., January 15, 2003; 63(2): 290 - 295.
[Abstract] [Full Text] [PDF]


Home page
Cancer Epidemiol. Biomarkers Prev.Home page
A. T. Baron, J. M. Lafky, V. J. Suman, D. W. Hillman, M. C. Buenafe, C. H. Boardman, K. C. Podratz, E. A. Perez, and N. J. Maihle
A Preliminary Study of Serum Concentrations of Soluble Epidermal Growth Factor Receptor (sErbB1), Gonadotropins, and Steroid Hormones in Healthy Men and Women
Cancer Epidemiol. Biomarkers Prev., November 1, 2001; 10(11): 1175 - 1185.
[Abstract] [Full Text] [PDF]


Home page
Cancer Res.Home page
S. M. Henshall, D. I. Quinn, C. S. Lee, D. R. Head, D. Golovsky, P. C. Brenner, W. Delprado, P. D. Stricker, J. J. Grygiel, and R. L. Sutherland
Altered Expression of Androgen Receptor in the Malignant Epithelium and Adjacent Stroma Is Associated with Early Relapse in Prostate Cancer
Cancer Res., January 1, 2001; 61(2): 423 - 427.
[Abstract] [Full Text]


Home page
Proc. Natl. Acad. Sci. USAHome page
D. K. Biswas, A. P. Cruz, E. Gansberger, and A. B. Pardee
Epidermal growth factor-induced nuclear factor kappa B activation: A major pathway of cell-cycle progression in estrogen-receptor negative breast cancer cells
PNAS, July 18, 2000; 97(15): 8542 - 8547.
[Abstract] [Full Text] [PDF]


Home page
J. Cell Sci.Home page
J. R. MacDougall and L. M. Matrisian
Targets of extinction: identification of genes whose expression is repressed as a consequence of somatic fusion between cells representing basal and luminal mammary epithelial phenotypes
J. Cell Sci., February 1, 2000; 113(3): 409 - 423.
[Abstract] [PDF]


Home page
Mol. Cell. Biol.Home page
O. W. J. Prall, E. M. Rogan, E. A. Musgrove, C. K. W. Watts, and R. L. Sutherland
c-Myc or Cyclin D1 Mimics Estrogen Effects on Cyclin E-Cdk2 Activation and Cell Cycle Reentry
Mol. Cell. Biol., August 1, 1998; 18(8): 4499 - 4508.
[Abstract] [Full Text] [PDF]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation
Copyright © 1997 by the American Association of Cancer Research.