CG&D
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Raptis, L.
Right arrow Articles by Haliotis, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Raptis, L.
Right arrow Articles by Haliotis, T.

Cell Growth & Differentiation, Vol 8, Issue 8 891-901, Copyright © 1997 by American Association of Cancer Research


ARTICLES

Cellular ras gene activity is required for full neoplastic transformation by the large tumor antigen of SV40

L Raptis, HL Brownell, MJ Corbley, KW Wood, D Wang and T Haliotis
Department of Microbiology and Immunology, Queen's University, Kingston, Ontario, Canada. raptisl@post.queensu.ca

To investigate the role of the cellular ras gene product in neoplastic transformation by the SV40 large tumor antigen (SVLT), murine C3H10T1/2 cells were rendered deficient in Ras activity by transfection with inducible or constitutive antisense ras gene constructs or through the introduction of the dominant-negative mutant, ras(asn17). Consistent with previous results, SVLT-induced morphological transformation was unaffected by the down-regulation of c-ras gene product activity. On the other hand, colony formation in soft agar and tumorigenicity in nude mice were drastically reduced in c-Ras-deficient cells. In addition, SVLT expression in C3H10T1/2 cells led to increased c-Ras activity, as determined by an increase in the Ras-bound GTP/GTP + GDP ratio. These results suggest that c-Ras is required for full neoplastic transformation by SVLT.


This article has been cited by other articles:


Home page
Clin. Cancer Res.Home page
B. F. Clem and J. Chesney
Molecular Pathways: Regulation of Metabolism by RB
Clin. Cancer Res., November 15, 2012; 18(22): 6096 - 6100.
[Abstract] [Full Text] [PDF]


Home page
Anticancer ResHome page
R. ARULANANDAM, M. GELETU, and L. RAPTIS
The Simian Virus 40 Large Tumor Antigen Activates cSrc and Requires cSrc for Full Neoplastic Transformation
Anticancer Res, January 1, 2010; 30(1): 47 - 53.
[Abstract] [Full Text] [PDF]


Home page
Mol. Cell. Biol.Home page
P. P. Fotiadou, C. Takahashi, H. N. Rajabi, and M. E. Ewen
Wild-Type NRas and KRas Perform Distinct Functions during Transformation
Mol. Cell. Biol., October 1, 2007; 27(19): 6742 - 6755.
[Abstract] [Full Text] [PDF]


Home page
Mol. Biol. CellHome page
A. Vultur, R. Arulanandam, J. Turkson, G. Niu, R. Jove, and L. Raptis
Stat3 Is Required for Full Neoplastic Transformation by the Simian Virus 40 Large Tumor Antigen
Mol. Biol. Cell, August 1, 2005; 16(8): 3832 - 3846.
[Abstract] [Full Text] [PDF]


Home page
Mol. Cell. Biol.Home page
C. Takahashi, B. Contreras, R. T. Bronson, M. Loda, and M. E. Ewen
Genetic Interaction between Rb and K-ras in the Control of Differentiation and Tumor Suppression
Mol. Cell. Biol., December 1, 2004; 24(23): 10406 - 10415.
[Abstract] [Full Text] [PDF]


Home page
Proc. Natl. Acad. Sci. USAHome page
N. Dube, A. Cheng, and M. L. Tremblay
The role of protein tyrosine phosphatase 1B in Ras signaling
PNAS, February 17, 2004; 101(7): 1834 - 1839.
[Abstract] [Full Text] [PDF]


Home page
Mol. Cell. Biol.Home page
C. Takahashi, R. T. Bronson, M. Socolovsky, B. Contreras, K. Y. Lee, T. Jacks, M. Noda, R. Kucherlapati, and M. E. Ewen
Rb and N-ras Function Together To Control Differentiation in the Mouse
Mol. Cell. Biol., August 1, 2003; 23(15): 5256 - 5268.
[Abstract] [Full Text] [PDF]


Home page
J Biol ChemHome page
N. Grammatikakis, A. Vultur, C. V. Ramana, A. Siganou, C. W. Schweinfest, D. K. Watson, and L. Raptis
The Role of Hsp90N, a New Member of the Hsp90 Family, in Signal Transduction and Neoplastic Transformation
J. Biol. Chem., March 8, 2002; 277(10): 8312 - 8320.
[Abstract] [Full Text] [PDF]


Home page
J Biol ChemHome page
N. Dourdin, A. K. Bhatt, P. Dutt, P. A. Greer, J. S. C. Arthur, J. S. Elce, and A. Huttenlocher
Reduced Cell Migration and Disruption of the Actin Cytoskeleton in Calpain-deficient Embryonic Fibroblasts
J. Biol. Chem., December 21, 2001; 276(51): 48382 - 48388.
[Abstract] [Full Text] [PDF]


Home page
J Biol ChemHome page
N. Grammatikakis, K. Jaronczyk, A. Siganou, A. Vultur, H. L. Brownell, M. Benzaquen, C. Rausch, R. Lapointe, O. Gjoerup, T. M. Roberts, et al.
Simian Virus 40 Large Tumor Antigen Modulates the Raf Signaling Pathway
J. Biol. Chem., July 27, 2001; 276(30): 27840 - 27845.
[Abstract] [Full Text] [PDF]


Home page
Cancer Res.Home page
I. Waheed, Z. S. Guo, G. A. Chen, T. S. Weiser, D. M. Nguyen, and D. S. Schrump
Antisense to SV40 Early Gene Region Induces Growth Arrest and Apoptosis in T-Antigen-positive Human Pleural Mesothelioma Cells
Cancer Res., December 1, 1999; 59(24): 6068 - 6073.
[Abstract] [Full Text] [PDF]


Home page
Mol. Cell. Biol.Home page
K. Y. Lee, M. H. Ladha, C. McMahon, and M. E. Ewen
The Retinoblastoma Protein Is Linked to the Activation of Ras
Mol. Cell. Biol., November 1, 1999; 19(11): 7724 - 7732.
[Abstract] [Full Text] [PDF]


Home page
J. Virol.Home page
V. Gottifredi, G. Pelicci, E. Munarriz, R. Maione, P. G. Pelicci, and P. Amati
Polyomavirus Large T Antigen Induces Alterations in Cytoplasmic Signalling Pathways Involving Shc Activation
J. Virol., February 1, 1999; 73(2): 1427 - 1437.
[Abstract] [Full Text] [PDF]


Home page
J Biol ChemHome page
N. Grammatikakis, K. Jaronczyk, A. Siganou, A. Vultur, H. L. Brownell, M. Benzaquen, C. Rausch, R. Lapointe, O. Gjoerup, T. M. Roberts, et al.
Simian Virus 40 Large Tumor Antigen Modulates the Raf Signaling Pathway
J. Biol. Chem., July 27, 2001; 276(30): 27840 - 27845.
[Abstract] [Full Text] [PDF]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation
Copyright © 1997 by the American Association of Cancer Research.