Cell Growth & Differentiation, Vol 8, Issue 7 811-820, Copyright © 1997 by American Association of Cancer Research

ARTICLES

Cyclin-dependent kinase activation and S-phase induction of the cyclin B1 gene are linked through the CCAAT elements

KS Katula, KL Wright, H Paul, DR Surman, FJ Nuckolls, JW Smith, JP Ting, J Yates and JP Cogswell
Department of Biology, University of North Carolina at Greensboro 27412, USA.

Control of cell proliferation is dependent on the regulated expression of the cyclin genes. Induction of cyclin B1 gene expression in S phase has been shown to require sequences within the first 90 bp of the proximal promoter region. In this study, we defined the cell cycle regulatory elements within this region and explored the mechanism by which the cyclin B1 gene is activated. A CDE-like element that is important in S-phase regulation of other genes was not required for correct cell cycle expression of cyclin B1. Instead, two CCAAT boxes were essential for S-phase induction of cyclin B1 gene in both NIH3T3 and HeLa cells. Induction of cyclin B1 by cyclin/cyclin-dependent kinase (cdk) complexes were examined by cotransfection of the reporter along with appropriate expression vectors. Complexes of cdk4 with cyclin D1 or cdk2 with cyclin E or A can activate the cyclin B1 promoter, and activation is uniquely dependent on the CCAAT elements in both normal and heterologous contexts. This transcription factor NF-Y binds to both CCAAT elements. These findings suggest that S phase-specific induction of the cyclin B1 promoter is dependent upon NF-Y binding to the CCAAT elements and is correlated with activation by cyclin-dependent kinases.

This article has been cited by other articles:

				S.-H. Park, G.-R. Yu, W.-H. Kim, W.-S. Moon, J.-H. Kim, and D.-G. Kim NF-Y-Dependent Cyclin B2 Expression in Colorectal Adenocarcinoma Clin. Cancer Res., February 1, 2007; 13(3): 858 - 867. [Abstract] [Full Text] [PDF]

				C. Chattopadhyay, D. Hawke, R. Kobayashi, and S. N. Maity Human p32, interacts with B subunit of the CCAAT-binding factor, CBF/NF-Y, and inhibits CBF-mediated transcription activation in vitro Nucleic Acids Res., July 8, 2004; 32(12): 3632 - 3641. [Abstract] [Full Text] [PDF]

				H. S. Yoon and V. W. Yang Requirement of Kruppel-like Factor 4 in Preventing Entry into Mitosis following DNA Damage J. Biol. Chem., February 6, 2004; 279(6): 5035 - 5041. [Abstract] [Full Text] [PDF]

				K. Himanen, C. Reuzeau, T. Beeckman, S. Melzer, O. Grandjean, L. Corben, and D. Inze The Arabidopsis Locus RCB Mediates Upstream Regulation of Mitotic Gene Expression Plant Physiology, December 1, 2003; 133(4): 1862 - 1872. [Abstract] [Full Text]

				J. P. H. Chow, W. Y. Siu, H. T. B. Ho, K. H. T. Ma, C. C. Ho, and R. Y. C. Poon Differential Contribution of Inhibitory Phosphorylation of CDC2 and CDK2 for Unperturbed Cell Cycle Control and DNA Integrity Checkpoints J. Biol. Chem., October 17, 2003; 278(42): 40815 - 40828. [Abstract] [Full Text] [PDF]

				J. Zhang, J. Yang, S. K. Roy, S. Tininini, J. Hu, J. F. Bromberg, V. Poli, G. R. Stark, and D. V. Kalvakolanu The cell death regulator GRIM-19 is an inhibitor of signal transducer and activator of transcription 3 PNAS, August 5, 2003; 100(16): 9342 - 9347. [Abstract] [Full Text] [PDF]

				K. Jurchott, S. Bergmann, U. Stein, W. Walther, M. Janz, I. Manni, G. Piaggio, E. Fietze, M. Dietel, and H.-D. Royer YB-1 as a Cell Cycle-regulated Transcription Factor Facilitating Cyclin A and Cyclin B1 Gene Expression J. Biol. Chem., July 18, 2003; 278(30): 27988 - 27996. [Abstract] [Full Text] [PDF]

				C. Borestrom, H. Zetterberg, K. Liff, and L. Rymo Functional Interaction of Nuclear Factor Y and Sp1 Is Required for Activation of the Epstein-Barr Virus C Promoter J. Virol., December 20, 2002; 77(2): 821 - 829. [Abstract] [Full Text] [PDF]

				R. M. Tjin Tham Sjin, K. Krishnaraju, B. Hoffman, and D. A. Liebermann Transcriptional regulation of myeloid differentiation primary response (MyD) genes during myeloid differentiation is mediated by nuclear factor Y Blood, June 17, 2002; 100(1): 80 - 88. [Abstract] [Full Text] [PDF]

				M. Ito, S. Araki, S. Matsunaga, T. Itoh, R. Nishihama, Y. Machida, J. H. Doonan, and A. Watanabe G2/M-Phase-Specific Transcription during the Plant Cell Cycle Is Mediated by c-Myb-Like Transcription Factors PLANT CELL, August 1, 2001; 13(8): 1891 - 1905. [Abstract] [Full Text] [PDF]

				L. A. Porter, G. Singh, and J. M. Lee Abundance of cyclin B1 regulates gamma -radiation-induced apoptosis Blood, April 15, 2000; 95(8): 2645 - 2650. [Abstract] [Full Text] [PDF]

				C. Badie, J. E. Itzhaki, M. J. Sullivan, A. J. Carpenter, and A. C. G. Porter Repression of CDK1 and Other Genes with CDE and CHR Promoter Elements during DNA Damage-Induced G2/M Arrest in Human Cells Mol. Cell. Biol., April 1, 2000; 20(7): 2358 - 2366. [Abstract] [Full Text]

				M. Park, H.-D. Chae, J. Yun, M. Jung, Y.-S. Kim, S.-H. Kim, M. H. Han, and D. Y. Shin Constitutive Activation of Cyclin B1-associated cdc2 Kinase Overrides p53-mediated G2-M Arrest Cancer Res., February 1, 2000; 60(3): 542 - 545. [Abstract] [Full Text]

				H. S. Radomska, A. B. Satterthwaite, N. Taranenko, S. Narravula, D. S. Krause, and D. G. Tenen A Nuclear Factor Y (NFY) Site Positively Regulates the Human CD34 Stem Cell Gene Blood, December 1, 1999; 94(11): 3772 - 3780. [Abstract] [Full Text] [PDF]

				W. R. Taylor, S. E. DePrimo, A. Agarwal, M. L. Agarwal, A. H. Schönthal, K. S. Katula, and G. R. Stark Mechanisms of G2 Arrest in Response to Overexpression of p53 Mol. Biol. Cell, November 1, 1999; 10(11): 3607 - 3622. [Abstract] [Full Text]

				P. Sorensen and E. Wintersberger Sp1 and NF-Y Are Necessary and Sufficient for Growth-dependent Regulation of the Hamster Thymidine Kinase Promoter J. Biol. Chem., October 22, 1999; 274(43): 30943 - 30949. [Abstract] [Full Text] [PDF]

				G. T. Park and M. I. Morasso Regulation of the Dlx3 Homeobox Gene upon Differentiation of Mouse Keratinocytes J. Biol. Chem., September 10, 1999; 274(37): 26599 - 26608. [Abstract] [Full Text] [PDF]

				S. A. Innocente, J. L. A. Abrahamson, J. P. Cogswell, and J. M. Lee p53 regulates a G2 checkpoint through cyclin B1 PNAS, March 2, 1999; 96(5): 2147 - 2152. [Abstract] [Full Text] [PDF]

				G. Marziali, E. Perrotti, R. Ilari, E. M. Coccia, R. Mantovani, U. Testa, and A. Battistini The Activity of the CCAAT-box Binding Factor NF-Y Is Modulated Through the Regulated Expression of Its A Subunit During Monocyte to Macrophage Differentiation: Regulation of Tissue-Specific Genes Through a Ubiquitous Transcription Factor Blood, January 15, 1999; 93(2): 519 - 526. [Abstract] [Full Text] [PDF]

				A. Hwang, W. G. McKenna, and R. J. Muschel Cell Cycle-dependent Usage of Transcriptional Start Sites. A NOVEL MECHANISM FOR REGULATION OF CYCLIN B1 J. Biol. Chem., November 20, 1998; 273(47): 31505 - 31509. [Abstract] [Full Text] [PDF]

				D. Kelly, S.-J. Kim, and A. Rizzino Transcriptional Activation of the Type II Transforming Growth Factor-beta Receptor Gene upon Differentiation of Embryonal Carcinoma Cells J. Biol. Chem., August 14, 1998; 273(33): 21115 - 21124. [Abstract] [Full Text] [PDF]

				F. Liang, F. Schaufele, and D. G. Gardner Functional Interaction of NF-Y and Sp1 Is Required for Type A Natriuretic Peptide Receptor Gene Transcription J. Biol. Chem., January 5, 2001; 276(2): 1516 - 1522. [Abstract] [Full Text] [PDF]