Cell Growth & Differentiation, Vol 8, Issue 7 789-800, Copyright © 1997 by American Association of Cancer Research

ARTICLES

Transcriptional regulation of intercellular adhesion molecule 1 by phorbol ester in human neuroblastoma cell line SK-N-SH involves jun- and fos-containing activator protein 1 site binding complex(es)

AR Farina, L Cappabianca, AR Mackay, A Tiberio, A Tacconelli, A Tessitore, L Frati, S Martinotti and A Gulino
Department of Experimental Medicine, University of L'Aquila, Italy.

In this study, the regulatory elements involved in ICAM-1 transcriptional response to phorbol ester (12-0-tetradecanoylphorbol-13-acetate; TPA) have been investigated in the human neuroblastoma cell line, SK-N-SH. TPA induced intercellular adhesion molecule 1 (ICAM-1) protein expression in SK-N-SH cells within 24 h of treatment as judged by indirect immunofluorescence. Basal ICAM-1 mRNA levels were barely detectable in untreated SK-N-SH cells but were induced by TPA to a maximal level with 4 h and were reduced thereafter. Analysis of the 5' promoter sequence of ICAM-1 revealed two regions that functioned equally in the TPA induction of ICAM-1 transcription. The first region (-145 to -227) contained a nuclear factor-kappa B (NF kappa B) element. The second region (-316 to -390) contained a putative TPA-responsive element (TRE; TGATTCA) and a TATA box. Deletion and point mutation of the latter region indicated that the TRE was indeed the functional element within this region and acted fully and independently of all other elements including the TATA box at position -352. This TRE bound TPA induced specific nuclear complexes in vitro containing junD, c-jun, c-fos, and fra2 but not cAMP-responsive element binding/activating transcription factor family proteins. ICAM-TRE binding activity was induced within 30 min following TPA treatment. This preceded the appearance of ICAM-NF kappa B site binding activity. Cotransfection of c-jun and c-fos expression vectors into SK-N-SH cells induced transactivation from ICAM-1 promoter constructs containing the intact but not mutated TRE site. Primer extension analyses revealed that TPA had induced transcription exclusively at two sites -40 and -41 bp upstream of the translation start site. These data show that the ICAM-TRE and its cognate jun- and fos-containing transcription factors play a predominant role in the transcriptional response of ICAM-1 to the protein kinase C activator TPA in SK-N-SH cells.

This article has been cited by other articles:

				R. G. Uzzo, P. L. Crispen, K. Golovine, P. Makhov, E. M. Horwitz, and V. M. Kolenko Diverse effects of zinc on NF-{kappa}B and AP-1 transcription factors: implications for prostate cancer progression Carcinogenesis, October 1, 2006; 27(10): 1980 - 1990. [Abstract] [Full Text] [PDF]

				Y.-J. Chang, M. J. Holtzman, and C.-C. Chen Interferon-gamma -induced Epithelial ICAM-1 Expression and Monocyte Adhesion. INVOLVEMENT OF PROTEIN KINASE C-DEPENDENT c-Src TYROSINE KINASE ACTIVATION PATHWAY J. Biol. Chem., February 22, 2002; 277(9): 7118 - 7126. [Abstract] [Full Text] [PDF]

				C. D. Kim, Y. K. Kim, S. H. Lee, and K. W. Hong Rebamipide Inhibits Neutrophil Adhesion to Hypoxia/Reoxygenation-Stimulated Endothelial Cells via Nuclear Factor-kappa B-Dependent Pathway J. Pharmacol. Exp. Ther., September 1, 2000; 294(3): 864 - 869. [Abstract] [Full Text]

				N. Wang, L. Verna, S. Hardy, J. Forsayeth, Y. Zhu, and M. B. Stemerman Adenovirus-Mediated Overexpression of c-Jun and c-Fos Induces Intercellular Adhesion Molecule-1 and Monocyte Chemoattractant Protein-1 in Human Endothelial Cells Arterioscler. Thromb. Vasc. Biol., September 1, 1999; 19(9): 2078 - 2084. [Abstract] [Full Text] [PDF]

				H. Kobuchi, S. Roy, C. K. Sen, H. G. Nguyen, and L. Packer Quercetin inhibits inducible ICAM-1 expression in human endothelial cells through the JNK pathway Am J Physiol Cell Physiol, September 1, 1999; 277(3): C403 - C411. [Abstract] [Full Text] [PDF]

				Q. Li, S. M. Vaingankar, H. M. Green, and M. Martins-Green Activation of the 9E3/cCAF Chemokine by Phorbol Esters Occurs via Multiple Signal Transduction Pathways That Converge to MEK1/ERK2 and Activate the Elk1 Transcription Factor J. Biol. Chem., May 28, 1999; 274(22): 15454 - 15465. [Abstract] [Full Text] [PDF]

				V. Baud, Z.-G. Liu, B. Bennett, N. Suzuki, Y. Xia, and M. Karin Signaling by proinflammatory cytokines: oligomerization of TRAF2 and TRAF6 is sufficient for JNK and IKK activation and target gene induction via an amino-terminal effector domain Genes & Dev., May 15, 1999; 13(10): 1297 - 1308. [Abstract] [Full Text]

				R. A. McKay, L. J. Miraglia, L. L. Cummins, S. R. Owens, H. Sasmor, and N. M. Dean Characterization of a Potent and Specific Class of Antisense Oligonucleotide Inhibitor of Human Protein Kinase C-alpha Expression J. Biol. Chem., January 15, 1999; 274(3): 1715 - 1722. [Abstract] [Full Text] [PDF]