CG&D
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bladier, C.
Right arrow Articles by Kola, I.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bladier, C.
Right arrow Articles by Kola, I.

Cell Growth & Differentiation, Vol 8, Issue 5 589-598, Copyright © 1997 by American Association of Cancer Research


ARTICLES

Response of a primary human fibroblast cell line to H2O2: senescence-like growth arrest or apoptosis?

C Bladier, EJ Wolvetang, P Hutchinson, JB de Haan and I Kola
Institute of Reproduction and Development, Monash University, Monash Medical Center, Clayton, Victoria, Australia.

Hydrogen peroxide has been shown to induce either apoptosis or features of senescence in different cultured cell lines. We now show that both processes can be induced in the same culture of primary human diploid fibroblasts and that the outcome of apoptosis or the senescence-like phenotype is determined by the H2O2 concentrations. At 50 and 100 microM, H2O2 predominantly induced the senescence-like state, characterized by a reduced rate of proliferation, an increased number of cells in G0-G1, typically enlarged and flattened morphology, and increased CIP1 and fibronectin expression. At 300 and 400 microM, H2O2 mainly triggered apoptosis. At the intermediate 200 microM H2O2, features of both senescence and apoptosis were observed in the same culture. Thus, the higher the H2O2 concentration, the higher the proportion of cells undergoing apoptosis, suggesting a key role of the level of damage in the choice of a cell population to enter apoptosis and/or the senescence-like state. Before the induction of one or the other process, cells entered a transient "shock state" characterized by a typical morphological change, cell cycle arrest in G0-G1, and the induction of CIP1 and BCL-2.


This article has been cited by other articles:


Home page
J Biol ChemHome page
C.-Y. Kan, C. Petti, L. Bracken, M. Maritz, N. Xu, R. O'Brien, C. Yang, T. Liu, J. Yuan, R. B. Lock, et al.
Up-regulation of Survivin during Immortalization of Human Myofibroblasts Is Linked to Repression of Tumor Suppressor p16INK4a Protein and Confers Resistance to Oxidative Stress
J. Biol. Chem., April 26, 2013; 288(17): 12032 - 12041.
[Abstract] [Full Text] [PDF]


Home page
Am. J. Physiol. Heart Circ. Physiol.Home page
J. Chen and M. S. Goligorsky
Premature senescence of endothelial cells: Methusaleh's dilemma
Am J Physiol Heart Circ Physiol, May 1, 2006; 290(5): H1729 - H1739.
[Abstract] [Full Text] [PDF]


Home page
Cancer Res.Home page
N. Miyoshi, K. Uchida, T. Osawa, and Y. Nakamura
A Link between Benzyl Isothiocyanate-Induced Cell Cycle Arrest and Apoptosis: Involvement of Mitogen-Activated Protein Kinases in the Bcl-2 Phosphorylation
Cancer Res., March 15, 2004; 64(6): 2134 - 2142.
[Abstract] [Full Text] [PDF]


Home page
J Biol ChemHome page
Y.-M. Go, J. J. Gipp, R. T. Mulcahy, and D. P. Jones
H2O2-dependent Activation of GCLC-ARE4 Reporter Occurs by Mitogen-activated Protein Kinase Pathways without Oxidation of Cellular Glutathione or Thioredoxin-1
J. Biol. Chem., February 13, 2004; 279(7): 5837 - 5845.
[Abstract] [Full Text] [PDF]


Home page
Cancer Res.Home page
D. Bernard, K. Gosselin, D. Monte, C. Vercamer, F. Bouali, A. Pourtier, B. Vandenbunder, and C. Abbadie
Involvement of Rel/Nuclear Factor-{kappa}B Transcription Factors in Keratinocyte Senescence
Cancer Res., January 15, 2004; 64(2): 472 - 481.
[Abstract] [Full Text] [PDF]


Home page
J. Leukoc. Biol.Home page
S. Kusmartsev and D. I. Gabrilovich
Inhibition of myeloid cell differentiation in cancer: the role of reactive oxygen species
J. Leukoc. Biol., August 1, 2003; 74(2): 186 - 196.
[Abstract] [Full Text] [PDF]


Home page
J Biol ChemHome page
J. H. Santos, L. Hunakova, Y. Chen, C. Bortner, and B. Van Houten
Cell Sorting Experiments Link Persistent Mitochondrial DNA Damage with Loss of Mitochondrial Membrane Potential and Apoptotic Cell Death
J. Biol. Chem., January 17, 2003; 278(3): 1728 - 1734.
[Abstract] [Full Text] [PDF]


Home page
Cancer Res.Home page
J. Fang, T. Sawa, T. Akaike, and H. Maeda
Tumor-targeted Delivery of Polyethylene Glycol-conjugated D-Amino Acid Oxidase for Antitumor Therapy via Enzymatic Generation of Hydrogen Peroxide
Cancer Res., June 1, 2002; 62(11): 3138 - 3143.
[Abstract] [Full Text] [PDF]


Home page
Physiol. Rev.Home page
W. Droge
Free Radicals in the Physiological Control of Cell Function
Physiol Rev, January 1, 2002; 82(1): 47 - 95.
[Abstract] [Full Text] [PDF]


Home page
J Biol ChemHome page
J. Duan, Z. Zhang, and T. Tong
Senescence Delay of Human Diploid Fibroblast Induced by Anti-sense p16INK4a Expression
J. Biol. Chem., December 21, 2001; 276(51): 48325 - 48331.
[Abstract] [Full Text] [PDF]


Home page
Proc. Natl. Acad. Sci. USAHome page
R. S. Arnold, J. Shi, E. Murad, A. M. Whalen, C. Q. Sun, R. Polavarapu, S. Parthasarathy, J. A. Petros, and J. D. Lambeth
Hydrogen peroxide mediates the cell growth and transformation caused by the mitogenic oxidase Nox1
PNAS, May 8, 2001; 98(10): 5550 - 5555.
[Abstract] [Full Text] [PDF]


Home page
Biol. Reprod.Home page
L. Liu, J. R. Trimarchi, and D. L. Keefe
Involvement of Mitochondria in Oxidative Stress-Induced Cell Death in Mouse Zygotes
Biol Reprod, June 1, 2000; 62(6): 1745 - 1753.
[Abstract] [Full Text]


Home page
Biol. Reprod.Home page
L. Liu and D. L. Keefe
Cytoplasm Mediates Both Development and Oxidation-Induced Apoptotic Cell Death in Mouse Zygotes
Biol Reprod, June 1, 2000; 62(6): 1828 - 1834.
[Abstract] [Full Text]


Home page
J Biol ChemHome page
M. Wartenberg, H. Diedershagen, J. Hescheler, and H. Sauer
Growth Stimulation Versus Induction of Cell Quiescence by Hydrogen Peroxide in Prostate Tumor Spheroids Is Encoded by the Duration of the Ca2+ Response
J. Biol. Chem., September 24, 1999; 274(39): 27759 - 27767.
[Abstract] [Full Text] [PDF]


Home page
J Biol ChemHome page
K. Tanabe, H. Nakanishi, H. Maeda, T. Nishioku, K. Hashimoto, S.-Y. Liou, A. Akamine, and K. Yamamoto
A Predominant Apoptotic Death Pathway of Neuronal PC12 Cells Induced by Activated Microglia Is Displaced by A Non-apoptotic Death Pathway Following Blockage of Caspase-3-dependent Cascade
J. Biol. Chem., May 28, 1999; 274(22): 15725 - 15731.
[Abstract] [Full Text] [PDF]


Home page
Infect. Immun.Home page
B. H. Segal, T. M. Doherty, T. A. Wynn, A. W. Cheever, A. Sher, and S. M. Holland
The p47phox-/- Mouse Model of Chronic Granulomatous Disease Has Normal Granuloma Formation and Cytokine Responses to Mycobacterium avium and Schistosoma mansoni Eggs
Infect. Immun., April 1, 1999; 67(4): 1659 - 1665.
[Abstract] [Full Text] [PDF]


Home page
J Biol ChemHome page
A. C. Lee, B. E. Fenster, H. Ito, K. Takeda, N. S. Bae, T. Hirai, Z.-X. Yu, V. J. Ferrans, B. H. Howard, and T. Finkel
Ras Proteins Induce Senescence by Altering the Intracellular Levels of Reactive Oxygen Species
J. Biol. Chem., March 19, 1999; 274(12): 7936 - 7940.
[Abstract] [Full Text] [PDF]


Home page
Am. J. Physiol. Cell Physiol.Home page
A. R. Simon, U. Rai, B. L. Fanburg, and B. H. Cochran
Activation of the JAK-STAT pathway by reactive oxygen species
Am J Physiol Cell Physiol, December 1, 1998; 275(6): C1640 - C1652.
[Abstract] [Full Text] [PDF]


Home page
J Biol ChemHome page
S. Simizu, M. Takada, K. Umezawa, and M. Imoto
Requirement of Caspase-3(-like) Protease-mediated Hydrogen Peroxide Production for Apoptosis Induced by Various Anticancer Drugs
J. Biol. Chem., October 9, 1998; 273(41): 26900 - 26907.
[Abstract] [Full Text] [PDF]


Home page
J Biol ChemHome page
J. B. de Haan, C. Bladier, P. Griffiths, M. Kelner, R. D. O'Shea, N. S. Cheung, R. T. Bronson, M. J. Silvestro, S. Wild, S. S. Zheng, et al.
Mice with a Homozygous Null Mutation for the Most Abundant Glutathione Peroxidase, Gpx1, Show Increased Susceptibility to the Oxidative Stress-inducing Agents Paraquat and Hydrogen Peroxide
J. Biol. Chem., August 28, 1998; 273(35): 22528 - 22536.
[Abstract] [Full Text] [PDF]


Home page
J Biol ChemHome page
V. M. Factor, A. Kiss, J. T. Woitach, P. J. Wirth, and S. S. Thorgeirsson
Disruption of Redox Homeostasis in the Transforming Growth Factor-{alpha}/c-myc Transgenic Mouse Model of Accelerated Hepatocarcinogenesis
J. Biol. Chem., June 19, 1998; 273(25): 15846 - 15853.
[Abstract] [Full Text] [PDF]


Home page
Proc. Natl. Acad. Sci. USAHome page
R. S. Arnold, J. Shi, E. Murad, A. M. Whalen, C. Q. Sun, R. Polavarapu, S. Parthasarathy, J. A. Petros, and J. D. Lambeth
Hydrogen peroxide mediates the cell growth and transformation caused by the mitogenic oxidase Nox1
PNAS, May 8, 2001; 98(10): 5550 - 5555.
[Abstract] [Full Text] [PDF]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation
Copyright © 1997 by the American Association of Cancer Research.