CG&D
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Conti, L.
Right arrow Articles by Gessani, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Conti, L.
Right arrow Articles by Gessani, S.

Cell Growth & Differentiation, Vol 8, Issue 4 435-442, Copyright © 1997 by American Association of Cancer Research


ARTICLES

Induction of relA(p65) and I kappa B alpha subunit expression during differentiation of human peripheral blood monocytes to macrophages

L Conti, J Hiscott, M Papacchini, A Roulston, MA Wainberg, F Belardelli and S Gessani
Laboratory of Virology, Istituto Superiore di Sanita, Rome, Italy.

We evaluated the expression and DNA binding activity of nuclear factor (NF)-kappa B subunits in human peripheral blood monocytes and in monocyte-derived macrophages (MDMs). Constitutive DNA binding activity consisting of p50 homodimers was detected in nuclear extracts from both cell types. An additional complex composed of p50/RelA(p65) heterodimers appeared only in nuclear extracts from 7-day MDMs. Immunoblot analysis showed that the p50 subunit was constitutively expressed in monocytes and MDMs. In contrast, the RelA(p65) subunit was barely detectable in monocytes, but its level increased markedly in MDMs. Analysis of RelA(p65) mRNA revealed that the stability of RelA(p65) mRNA was significantly higher in MDMs, compared with monocytes. In MDMs, an upregulation of I kappa B alpha synthesis as well as the appearance of a novel M(r) 40,000 form of I kappa B alpha were also observed. These results suggest that macrophage differentiation results in the expression of active p50/RelA(p65) heterodimers with the capacity to activate target gene expression. The parallel induction of I kappa B alpha synthesis may allow for the continuous presence of a cytoplasmic reservoir of p50/RelA(p65) complexes that are readily available for inducer-mediated stimulation.


This article has been cited by other articles:


Home page
FASEB J.Home page
N. Tang, B. Sun, A. Gupta, H. Rempel, and L. Pulliam
Monocyte exosomes induce adhesion molecules and cytokines via activation of NF-{kappa}B in endothelial cells
FASEB J, September 1, 2016; 30(9): 3097 - 3106.
[Abstract] [Full Text] [PDF]


Home page
J. Pharmacol. Exp. Ther.Home page
A. Lemarie, C. Morzadec, D. Merino, O. Micheau, O. Fardel, and L. Vernhet
Arsenic Trioxide Induces Apoptosis of Human Monocytes during Macrophagic Differentiation through Nuclear Factor-{kappa}B-Related Survival Pathway Down-Regulation
J. Pharmacol. Exp. Ther., January 1, 2006; 316(1): 304 - 314.
[Abstract] [Full Text] [PDF]


Home page
J. Immunol.Home page
H. Liu, Y. Ma, L. J. Pagliari, H. Perlman, C. Yu, A. Lin, and R. M. Pope
TNF-{alpha}-Induced Apoptosis of Macrophages Following Inhibition of NF-{kappa}B: A Central Role for Disruption of Mitochondria
J. Immunol., February 1, 2004; 172(3): 1907 - 1915.
[Abstract] [Full Text] [PDF]


Home page
JEMHome page
S. Shi, C. Nathan, D. Schnappinger, J. Drenkow, M. Fuortes, E. Block, A. Ding, T. R. Gingeras, G. Schoolnik, S. Akira, et al.
MyD88 Primes Macrophages for Full-Scale Activation by Interferon-{gamma} yet Mediates Few Responses to Mycobacterium tuberculosis
J. Exp. Med., October 6, 2003; 198(7): 987 - 997.
[Abstract] [Full Text] [PDF]


Home page
BloodHome page
F. Bureau, A. Vanderplasschen, F. Jaspar, F. Minner, P.-P. Pastoret, M.-P. Merville, V. Bours, and P. Lekeux
Constitutive nuclear factor-kappa B activity preserves homeostasis of quiescent mature lymphocytes and granulocytes by controlling the expression of distinct Bcl-2 family proteins
Blood, May 15, 2002; 99(10): 3683 - 3691.
[Abstract] [Full Text] [PDF]


Home page
J. Immunol.Home page
T. Kawashima, K. Murata, S. Akira, Y. Tonozuka, Y. Minoshima, S. Feng, H. Kumagai, H. Tsuruga, Y. Ikeda, S. Asano, et al.
STAT5 Induces Macrophage Differentiation of M1 Leukemia Cells Through Activation of IL-6 Production Mediated by NF-{kappa}B p65
J. Immunol., October 1, 2001; 167(7): 3652 - 3660.
[Abstract] [Full Text] [PDF]


Home page
J. Immunol.Home page
H. A. Smits, A. J. van Beelen, N. M. de Vos, A. Rijsmus, T. van der Bruggen, J. Verhoef, F. L. van Muiswinkel, and H. S. L. M. Nottet
Activation of Human Macrophages by Amyloid-{beta} Is Attenuated by Astrocytes
J. Immunol., June 1, 2001; 166(11): 6869 - 6876.
[Abstract] [Full Text] [PDF]


Home page
Mol. Cell. Biol.Home page
L. J. Pagliari, H. Perlman, H. Liu, and R. M. Pope
Macrophages Require Constitutive NF-kappa B Activation To Maintain A1 Expression and Mitochondrial Homeostasis
Mol. Cell. Biol., December 1, 2000; 20(23): 8855 - 8865.
[Abstract] [Full Text] [PDF]


Home page
J. Leukoc. Biol.Home page
L. Fantuzzi, L. Conti, M. C. Gauzzi, P. Eid, M. Del Corno, B. Varano, I. Canini, F. Belardelli, and S. Gessani
Regulation of chemokine/cytokine network during in vitro differentiation and HIV-1 infection of human monocytes: possible importance in the pathogenesis of AIDS
J. Leukoc. Biol., September 1, 2000; 68(3): 391 - 399.
[Abstract] [Full Text] [PDF]


Home page
J. Immunol.Home page
T. D. Camenisch, B. H. Koller, H. S. Earp2, and G. K. Matsushima
A Novel Receptor Tyrosine Kinase, Mer, Inhibits TNF-{alpha} Production and Lipopolysaccharide-Induced Endotoxic Shock
J. Immunol., March 15, 1999; 162(6): 3498 - 3503.
[Abstract] [Full Text] [PDF]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation
Copyright © 1997 by the American Association of Cancer Research.