Cell Growth & Differentiation, Vol 8, Issue 11 1181-1188, Copyright © 1997 by American Association of Cancer Research

ARTICLES

Sustained versus transient cyclic AMP intracellular levels: effect on thyrotropin-dependent growth of thyroid cells

G Villone, F De Amicis, BM Veneziani, S Salzano, A Di Carlo and D Tramontano
Dipartimento di Medicina Sperimentale e Clinica, Universita di Reggio Calabria, Catanzaro, Italy.

Cyclic AMP (cAMP) is the second messenger that stimulates growth and differentiation of thyroid cells, which are dependent upon thyrotropin for the initiation of the cell cycle. Treatment of thyroid cells with phosphodiesterase inhibitors, such as 1-methyl-3-isobutylxanthine (IBMX), RO-20-1724, or aminophylline, induces persistent levels of cAMP and blocks cell proliferation. IBMX-treated cells are arrested at the G1-S border, but removal of the drug allows cell growth to resume. The inhibiting effect of IBMX is dose dependent, and the phase of the cell cycle is irrelevant. These data indicate that prolonged and steady accumulation of cAMP blocks the cell cycle in thyroid cells.

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