Cell Growth & Differentiation, Vol 8, Issue 10 1099-1104, Copyright © 1997 by American Association of Cancer Research

ARTICLES

Differential regulation of transcription of p21 and cyclin D1 conferred by TAF(II)250

JJ Rushton, RA Steinman and PD Robbins
Department of Molecular Genetics and Biochemistry, University of Pittsburgh, School of Medicine, Pennsylvania 15261, USA.

The TATA-binding protein-associated factor TAF(II)250, the largest subunit of TFIID, was first identified as the cell cycle regulatory protein CCG1. The ts13 Syrian hamster ovary fibroblast cell line, which contains a temperature-sensitive point mutation in TAF(II)250/CCG1, is arrested in G1 following a shift to the nonpermissive temperature. Here we demonstrate that the level of the D-type cyclins, in particular D1, was reduced, whereas the level of the cyclin-dependent kinase inhibitor p21 was stimulated in ts13 cells at the nonpermissive temperature. The levels of expression of cyclins A and E were not affected by temperature shift. We further show that at least part of the regulation of D1 and p21 levels in ts13 cells is mediated at the level of transcription initiation. These results suggest that the effect of the temperature-sensitive mutation TAF(II)250 on cell growth can be mediated through the differential regulation of transcription of specific cell growth regulatory genes, such as cyclin D1 and p21.

This article has been cited by other articles:

				T. L. Hilton, Y. Li, E. L. Dunphy, and E. H. Wang TAF1 Histone Acetyltransferase Activity in Sp1 Activation of the Cyclin D1 Promoter Mol. Cell. Biol., May 15, 2005; 25(10): 4321 - 4332. [Abstract] [Full Text] [PDF]

				A. M. Buchmann, J. R. Skaar, and J. A. DeCaprio Activation of a DNA Damage Checkpoint Response in a TAF1-Defective Cell Line Mol. Cell. Biol., June 15, 2004; 24(12): 5332 - 5339. [Abstract] [Full Text] [PDF]

				S. M. Dehm, T. L. Hilton, E. H. Wang, and K. Bonham SRC Proximal and Core Promoter Elements Dictate TAF1 Dependence and Transcriptional Repression by Histone Deacetylase Inhibitors Mol. Cell. Biol., March 15, 2004; 24(6): 2296 - 2307. [Abstract] [Full Text] [PDF]

				C. Wasylyk and B. Wasylyk Defect in the p53-Mdm2 Autoregulatory Loop Resulting from Inactivation of TAFII250 in Cell Cycle Mutant tsBN462 Cells Mol. Cell. Biol., August 1, 2000; 20(15): 5554 - 5570. [Abstract] [Full Text]

				E. L. Dunphy, T. Johnson, S. S. Auerbach, and E. H. Wang Requirement for TAFII250 Acetyltransferase Activity in Cell Cycle Progression Mol. Cell. Biol., February 15, 2000; 20(4): 1134 - 1139. [Abstract] [Full Text]

				J. L. Siegert and P. D. Robbins Rb Inhibits the Intrinsic Kinase Activity of TATA-Binding Protein-Associated Factor TAFII250 Mol. Cell. Biol., January 1, 1999; 19(1): 846 - 854. [Abstract] [Full Text] [PDF]