Cell Growth & Differentiation, Vol 7, Issue 12 1761-1767, Copyright © 1996 by American Association of Cancer Research
Involvement of gicerin, a cell adhesion molecule, in tracheal development and regeneration
Y Tsukamoto, E Taira, T Kotani, J Yamate, S Wada, N Takaha, N Miki and S Sakuma
Department of Veterinary Pathology, College of Agriculture, Osaka Prefecture University, Japan.
Gicerin is a novel cell adhesion protein that belongs to the immunoglobulin
superfamily. Gicerin protein adheres to neurite outgrowth factor, an
extracellular matrix protein in the laminin family, and also exhibits
homophilic adhesion. In the present study, we investigated the involvement
of gicerin and neurite outgrowth factor in tracheal development and
regeneration. In an early embryonic stage, gicerin protein was highly
expressed in tracheal epithelial cells, but not in loosely arranged
mesenchymal cells. During development, mesenchymal cells become condensed
around the tracheal epithelium and then differentiate into muscle and
cartilage; high levels of gicerin expression were observed in these cells.
In the later embryonic and posthatching stages, no gicerin expression was
detected in tracheal epithelium or cartilage. In addition, expression of
gicerin increased transiently in the tracheal epithelium during the
regeneration after tracheitis induced by the infectious bronchitis virus.
Furthermore, a polyclonal antibody against gicerin inhibited the epithelial
regeneration in tracheal organ cultures. These findings suggest that
glcerin plays an important role in both tracheal development and