CG&D
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Chen, Y.
Right arrow Articles by Conti, C. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chen, Y.
Right arrow Articles by Conti, C. J.

Cell Growth & Differentiation, Vol 7, Issue 11 1571-1578, Copyright © 1996 by American Association of Cancer Research


ARTICLES

Expression of G1 cyclins, cyclin-dependent kinases, and cyclin-dependent kinase inhibitors in androgen-induced prostate proliferation in castrated rats

Y Chen, AI Robles, LA Martinez, F Liu, IB Gimenez-Conti and CJ Conti
University of Texas M. D. Anderson Cancer Center, Science Park-Research Division, Smithville 78957, USA.

Androgen induces prostate cell proliferation in the castrated rat. We hypothesized that G1 cyclins, cyclin-dependent kinases (cdk), and cdk inhibitors mediate this cellular response to mitogenic signals. In this study, induction of cyclins D1, D2, D3, E, and cdks 2, 4, and 6 expression was observed at various time points during testosterone replacement in the ventral prostate of castrated rats. The induction followed prostate epithelium proliferation, which peaked at 48 h and decreased at 120 h during the treatment. The study of cyclin/cdk complex formation revealed that more cyclin D1/cdk4 and cyclin D1/cdk6 complexes were formed at 48 h than at 120 h of treatment, but cyclin D1/cdk2 complexes remained the same. Furthermore, both hyperphosphorylated and hypophosphorylated forms of Rb were detected at 48 h, but only the hypophosphorylated form was detected at 120 h of treatment. p21Cip1, which was very abundant in the ventral prostate of castrated and intact rats, was not detected when the prostate started proliferation and increased gradually as proliferation decreased during the androgen treatment. Meanwhile, p27Kip1 dramatically increased after androgen treatment, and the induction levels were less at the peak of prostate proliferation and higher when proliferation was low. The results presented here suggest that expression of G1 cyclins and their related kinases and kinase inhibitors are well regulated after androgen replacement in the ventral prostate of castrated rats. The cooperation between these cell cycle regulators leads to a well-controlled prostate regeneration.


This article has been cited by other articles:


Home page
Cancer Res.Home page
G. N. Brooke, R. L. Culley, D. A. Dart, D. J. Mann, L. Gaughan, S. R. McCracken, C. N. Robson, B. Spencer-Dene, S. C. Gamble, S. M. Powell, et al.
FUS/TLS Is a Novel Mediator of Androgen-Dependent Cell-Cycle Progression and Prostate Cancer Growth
Cancer Res., February 1, 2011; 71(3): 914 - 924.
[Abstract] [Full Text] [PDF]


Home page
Endocr Relat CancerHome page
H. Neuwirt, M. Puhr, I. T Cavarretta, M. Mitterberger, A. Hobisch, and Z. Culig
Suppressor of cytokine signalling-3 is up-regulated by androgen in prostate cancer cell lines and inhibits androgen-mediated proliferation and secretion
Endocr. Relat. Cancer, December 1, 2007; 14(4): 1007 - 1019.
[Abstract] [Full Text] [PDF]


Home page
Cancer Res.Home page
T. I. Klokk, A. Kilander, Z. Xi, H. Waehre, B. Risberg, H. E. Danielsen, and F. Saatcioglu
Kallikrein 4 Is a Proliferative Factor that Is Overexpressed in Prostate Cancer
Cancer Res., June 1, 2007; 67(11): 5221 - 5230.
[Abstract] [Full Text] [PDF]


Home page
Cancer Res.Home page
Y. Xu, S.-Y. Chen, K. N. Ross, and S. P. Balk
Androgens Induce Prostate Cancer Cell Proliferation through Mammalian Target of Rapamycin Activation and Post-transcriptional Increases in Cyclin D Proteins.
Cancer Res., August 1, 2006; 66(15): 7783 - 7792.
[Abstract] [Full Text] [PDF]


Home page
ReproductionHome page
W. H Walker and J. Cheng
FSH and testosterone signaling in Sertoli cells
Reproduction, July 1, 2005; 130(1): 15 - 28.
[Abstract] [Full Text] [PDF]


Home page
J Biol ChemHome page
L. Zhang and C. Wang
PAX3-FKHR Transformation Increases 26 S Proteasome-dependent Degradation of p27Kip1, a Potential Role for Elevated Skp2 Expression
J. Biol. Chem., January 3, 2003; 278(1): 27 - 36.
[Abstract] [Full Text] [PDF]


Home page
J Biol ChemHome page
K. C. Day, M. T. McCabe, X. Zhao, Y. Wang, J. N. Davis, J. Phillips, M. Von Geldern, T. Ried, M. A. KuKuruga, G. R. Cunha, et al.
Rescue of Embryonic Epithelium Reveals That the Homozygous Deletion of the Retinoblastoma Gene Confers Growth Factor Independence and Immortality but Does Not Influence Epithelial Differentiation or Tissue Morphogenesis
J. Biol. Chem., November 15, 2002; 277(46): 44475 - 44484.
[Abstract] [Full Text] [PDF]


Home page
J Biol ChemHome page
L. Xia, D. Robinson, A.-H. Ma, H.-C. Chen, F. Wu, Y. Qiu, and H.-J. Kung
Identification of Human Male Germ Cell-associated Kinase, a Kinase Transcriptionally Activated by Androgen in Prostate Cancer Cells
J. Biol. Chem., September 20, 2002; 277(38): 35422 - 35433.
[Abstract] [Full Text] [PDF]


Home page
Cell Growth Differ.Home page
A. Tamir, T. Petrocelli, K. Stetler, W. Chu, J. Howard, B. St. Croix, J. Slingerland, and Y. Ben-David
Stem Cell Factor Inhibits Erythroid Differentiation by Modulating the Activity of G1-Cyclin-dependent Kinase Complexes: A Role for p27 in Erythroid Differentiation Coupled G1 Arrest
Cell Growth Differ., May 1, 2000; 11(5): 269 - 277.
[Abstract] [Full Text]


Home page
Mol. Pathol.Home page
Z Kolar, P G Murray, K Scott, A Harrison, B Vojtesek, and J Dusek
Relation of Bcl-2 expression to androgen receptor, p21WAF1/CIP1, and cyclin D1 status in prostate cancer
Mol. Pathol., February 1, 2000; 53(1): 15 - 18.
[Abstract] [Full Text]


Home page
Proc. Natl. Acad. Sci. USAHome page
X. Zi and R. Agarwal
Silibinin decreases prostate-specific antigen with cell growth inhibition via G1 arrest, leading to differentiation of prostate carcinoma cells: Implications for prostate cancer intervention
PNAS, June 22, 1999; 96(13): 7490 - 7495.
[Abstract] [Full Text] [PDF]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation
Copyright © 1996 by the American Association of Cancer Research.