Cell Growth & Differentiation, Vol 6, Issue 6 719-726, Copyright © 1995 by American Association of Cancer Research

ARTICLES

Mitogen induction of nuclear factors that interact with a delayed responsive region of the transferrin receptor gene promoter

S Hirsch and WK Miskimins
Department of Biochemistry and Molecular Biology, University of South Dakota, School of Medicine, Vermillion 57069, USA.

Activation of the human transferrin receptor promoter by mitogenic stimulation of quiescent cells is a delayed event that reaches a maximum several hours after stimulation. Previous results have defined a region of the transferrin receptor gene promoter that is required for increased expression in mitogen-activated cells (W. K. Miskimins and D. B. Brown, Exp. Cell Res., 191: 328-331, 1990; Q. Ouyang et al., Mol. Cell. Biol., 13: 1796-1804, 1993). This region contains two elements (elements A and B) that appear to cooperate in the response to mitogenic stimulation. Serum stimulation of quiescent cells leads to the induction of nuclear factors that bind to both the A and B elements. Induction of these factors is also a delayed response to serum stimulation and reaches a maximum 6-9 h after stimulation. Element A, which is an unusual GC-rich sequence, forms several serum-inducible DNA-protein complexes, all of which depend on contacts within GC boxes. A major inducible complex of element A contains a factor that is supershifted by antibodies against the transcription factor Sp1. The B element appears to have overlapping binding sites for two types of factors. One of these sites binds factors that are competed off by an AP-1 consensus-binding site. The other B element site binds inducible factors that interact with GC boxes, identical to those observed for element A.

This article has been cited by other articles:

				O. Stehling, D. J. A. Netz, B. Niggemeyer, R. Rosser, R. S. Eisenstein, H. Puccio, A. J. Pierik, and R. Lill Human Nbp35 Is Essential for both Cytosolic Iron-Sulfur Protein Assembly and Iron Homeostasis Mol. Cell. Biol., September 1, 2008; 28(17): 5517 - 5528. [Abstract] [Full Text] [PDF]

				J.-Y. Li, G. Ram, K. Gast, X. Chen, K. Barasch, K. Mori, K. Schmidt-Ott, J. Wang, H.-C. Kuo, C. Savage-Dunn, et al. Detection of intracellular iron by its regulatory effect Am J Physiol Cell Physiol, December 1, 2004; 287(6): C1547 - C1559. [Abstract] [Full Text] [PDF]

				L. Tacchini, L. Bianchi, A. Bernelli-Zazzera, and G. Cairo Transferrin Receptor Induction by Hypoxia. HIF-1-MEDIATED TRANSCRIPTIONAL ACTIVATION AND CELL-SPECIFIC POST-TRANSCRIPTIONAL REGULATION J. Biol. Chem., August 20, 1999; 274(34): 24142 - 24146. [Abstract] [Full Text] [PDF]